Document Detail

Identification and characterization of developmentally regulated genes in vascular smooth muscle cells.
MedLine Citation:
PMID:  1339321     Owner:  NLM     Status:  MEDLINE    
We wish to understand the process of smooth muscle cell (SMC) proliferation and maturation during late fetal development and have examined some of the molecular changes associated with blood vessel maturation in late gestational and early neonatal life. By differential screening of a fetal aortic smooth muscle cDNA library, we identified a gene (F-31) that was developmentally regulated in aortic smooth muscle. The F-31 gene encodes a 2.3-kb RNA that was highly expressed in fetal aortic smooth muscle (25-day gestation), was lower in newborns, and was undetectable in the aortic smooth muscle of 4-week-old animals. F-31 was also highly expressed in fetal muscle, esophagus, heart, liver, lung, and placenta; its expression was lower in skin, kidney, and brain. By contrast, the expression of F-31 was low or undetectable in the corresponding tissue of adult animals. DNA sequence analysis of cDNAs encoding F-31 and data base comparison revealed a 73% homology with a previously identified, developmentally regulated gene called H19. We also found that insulin-like growth factor II (IGF-II) expression was developmentally regulated in smooth muscle. However, unlike F-31, expression of IGF-II was undetectable in the aortic smooth muscle of newborn animals. Analysis of the mRNA level of several genes that encode cytoskeletal proteins in neonatal, newborn, and adult smooth muscle indicates that total actin mRNA level, alpha-smooth muscle actin, and alpha-tropomyosin mRNA levels were similar between the late gestational period and 4 weeks after birth.(ABSTRACT TRUNCATED AT 250 WORDS)
D K Han; G Liau
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation research     Volume:  71     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-09-17     Completed Date:  1992-09-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  711-9     Citation Subset:  IM    
Department of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, Md 20855.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M81348;  M83092;  M97348;  S47168;  S49998;  S50001;  S72771;  X64094;  X64095;  X64096
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MeSH Terms
Amino Acid Sequence
Animals, Newborn
Aorta / metabolism
Base Sequence
Cells, Cultured
Cloning, Molecular
Gene Expression Regulation
Genes / genetics*
Gestational Age
Insulin-Like Growth Factor II / genetics*
Molecular Sequence Data
Morphogenesis / genetics
Muscle, Smooth, Vascular / metabolism*
Grant Support
Reg. No./Substance:
67763-97-7/Insulin-Like Growth Factor II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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