Document Detail


Identification and characterization of a conserved, stage-specific gene product of Plasmodium falciparum recognized by parasite growth inhibitory antibodies.
MedLine Citation:
PMID:  12654839     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have identified a novel conserved protein of Plasmodium falciparum, designated D13, that is stage-specifically expressed in asexual blood stages of the parasite. The predicted open reading frame (ORF) D13 contains 863 amino acids with a calculated molecular mass of 99.7 kDa and displays a repeat region composed of pentapeptide motives. Northern blot analysis with lysates of synchronized blood stage parasites showed that D13 is highly expressed at the mRNA level during schizogony. The first N'-terminal 138 amino acids of D13 were expressed in Escherichia coli and the purified protein was used to generate anti-D13 monoclonal antibodies (MAbs). Using total lysates of blood stage parasites and Western blot analysis, these MAbs stained one single band of approximately 100 kDa, corresponding to the predicted molecular mass of ORF D13. Western blot analysis demonstrated further that D13 is expressed during schizogony, declines rapidly in early ring stages and is undetectable in trophozoites. D13 protein is localized in individual merozoites in a distinct area, as demonstrated by indirect immunofluorescence analysis. After subcellular fractionation, D13 was confined to the pelleted fraction of the parasite lysate and its extraction by alkaline carbonate buffer treatment indicated that D13 is not a membrane-integral protein. Inclusion of certain anti-D13 MAbs into in vitro cultures of blood stage parasites resulted in considerable reduction in parasite growth. The N'-terminal domain encompassing 158 amino acids is 94 and 95%, respectively, identical at the amino acid level between Plasmodium knowlesi, Plasmodium yoelii, and P. falciparum. In summary, we describe a novel stage-specifically expressed, highly conserved gene product of P. falciparum that is recognized by parasite growth inhibitory antibodies.
Authors:
Claudia A Daubenberger; Diana Diaz; Marija Curcic; Markus S Mueller; Tobias Spielmann; Ulrich Certa; Joachim Lipp; Gerd Pluschke
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  71     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-25     Completed Date:  2003-05-12     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2173-81     Citation Subset:  IM    
Affiliation:
Molecular Immunology, Swiss Tropical Institute, Socinstrasse 57, 4002 Basel, Switzerland. Claudia.Daubenberger@unibas.ch
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF491296;  AF491297;  AF491298
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Monoclonal / immunology
Antibodies, Protozoan / immunology*
Erythrocytes / parasitology*
Humans
Life Cycle Stages
Malaria, Falciparum / parasitology*
Mice
Molecular Sequence Data
Open Reading Frames
Plasmodium falciparum / growth & development*,  immunology
Protozoan Proteins / genetics*,  immunology,  metabolism*
Sequence Analysis, DNA
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Protozoan; 0/D13 protein, Plasmodium falciparum; 0/Protozoan Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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