Document Detail


Identification and characterization of a complete carnitine biosynthesis pathway in Candida albicans.
MedLine Citation:
PMID:  19289605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carnitine is an essential metabolite that enables intracellular transport of fatty acids and acetyl units. Here we show that the yeast Candida albicans can synthesize carnitine de novo, and we identify the 4 genes of the pathway. Null mutants of orf19.4316 (trimethyllysine dioxygenase), orf19.6306 (trimethylaminobutyraldehyde dehydrogenase), and orf19.7131 (butyrobetaine dioxygenase) lacked their respective enzymatic activities and were unable to utilize fatty acids, acetate, or ethanol as a sole carbon source, in accordance with the strict requirement for carnitine-mediated transport under these growth conditions. The second enzyme of carnitine biosynthesis, hydroxytrimethyllysine aldolase, is encoded by orf19.6305, a member of the threonine aldolase (TA) family in C. albicans. A strain lacking orf19.6305 showed strongly reduced growth on fatty acids and was unable to utilize either acetate or ethanol, but TA activity was unaffected. Growth of the null mutants on nonfermentable carbon sources is restored only by carnitine biosynthesis intermediates after the predicted enzymatic block in the pathway, which provides independent evidence for a specific defect in carnitine biosynthesis for each of the mutants. In conclusion, we have genetically characterized a complete carnitine biosynthesis pathway in C. albicans and show that a TA family member is mainly involved in the aldolytic cleavage of hydroxytrimethyllysine in vivo.
Authors:
Karin Strijbis; Carlo W T van Roermund; Guy P Hardy; Janny van den Burg; Karien Bloem; Jolanda de Haan; Naomi van Vlies; Ronald J A Wanders; Frédéric M Vaz; Ben Distel
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Publication Detail:
Type:  Journal Article     Date:  2009-03-16
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  23     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-31     Completed Date:  2009-08-18     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2349-59     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Aldehyde Oxidoreductases / genetics,  metabolism
Aldehyde-Lyases / genetics,  metabolism
Amino Acid Sequence
Candida albicans / genetics,  growth & development,  metabolism*
Carnitine / biosynthesis*,  chemistry
Genes, Fungal
Kinetics
Mixed Function Oxygenases / genetics,  metabolism
Models, Biological
Molecular Sequence Data
Mutation
Sequence Homology, Amino Acid
gamma-Butyrobetaine Dioxygenase / genetics,  metabolism
Chemical
Reg. No./Substance:
541-15-1/Carnitine; EC 1.-/Mixed Function Oxygenases; EC 1.14.11.1/gamma-Butyrobetaine Dioxygenase; EC 1.14.11.8/epsilon-N-trimethyllysine hydroxylase; EC 1.2.-/4-N-trimethylaminobutyraldehyde dehydrogenase; EC 1.2.-/Aldehyde Oxidoreductases; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.-/beta-hydroxy-trimethyllysine aldolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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