Document Detail


Identification and characterization of autocrine-motility-factor-like activity in oral squamous-cell-carcinoma cells.
MedLine Citation:
PMID:  7989119     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human oral squamous-cell-carcinoma cell line, HOC313, was found to produce a factor which stimulates cell motility in an autocrine manner. The motility factor of HOC313 cells also promoted the locomotory activity of B16 murine melanoma cells reported to be sensitive to autocrine motility factor (AMF). HOC313 cells were found to express a large amount of AMF-receptor mRNA. In addition, the cell motility activity of HOC313 cells was completely blocked by pertussis toxin, a known inhibitor of AMF activity, suggesting that the motility factor of HOC313 cells may be AMF or a closely related factor. Immunocytochemical analysis has revealed that the AMF-like factor of HOC313 cells diminishes the cell-surface expression of adhesive molecule E-cadherin. These results suggest that down-regulation of E-cadherin may be involved in the cell-motility activity induced by the AMF-like factor of HOC313 cells.
Authors:
A Ishisaki; S Oida; F Momose; T Amagasa; K Rikimaru; H Ichijo; S Sasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  59     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-01-12     Completed Date:  1995-01-12     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  783-8     Citation Subset:  IM    
Affiliation:
Department of Oral and Maxillofacial Surgery, Tokyo Medical and Dental University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autocrine Motility Factor / analysis*,  antagonists & inhibitors,  pharmacology
Cadherins / analysis
Carcinoma, Squamous Cell / chemistry*,  pathology
Cell Movement / drug effects
Chromatography, Gel
Culture Media, Conditioned
Humans
Melanoma, Experimental / pathology
Mice
Mouth Neoplasms / chemistry*,  pathology
Pertussis Toxin
RNA, Messenger / analysis
RNA, Neoplasm / analysis
Tumor Cells, Cultured
Virulence Factors, Bordetella / pharmacology
Chemical
Reg. No./Substance:
0/Autocrine Motility Factor; 0/Cadherins; 0/Culture Media, Conditioned; 0/RNA, Messenger; 0/RNA, Neoplasm; 0/Virulence Factors, Bordetella; EC 2.4.2.31/Pertussis Toxin

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