Document Detail


Identification and characterization of antigenic epitope of Staphylococcus aureus ClfA adhesin.
MedLine Citation:
PMID:  23178048     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Staphylococcus aureus ClfA adhesin is a protective antigen that induces partial immunity against S. aureus infection in mice. To identify the antigenic epitope of ClfA, a monoclonal antibody (mAb) D01 against the recombinant protein was produced by the hybridoma technique. The mAb was used to immunoscreen a random phage-displayed peptide library as the immunogen. After three rounds of biopanning, 41 positive clones were identified. Sixteen phage clones were sequenced and their amino acids were deduced. One mimotope (SKVGIDKRRGTA) showed good match with ClfA adhesin at 383-394 aa and the serum of mice induced by the phage clone clearly recognized ClfA adhesin.
Authors:
Yang Li; Ya-Hui Liu; Zhi-Jie Li; Ya-Gang Li; Ming-Yuan Liu; Li-Bo Liu; Xiao-Guang Wang; Xue-Lin Wang; Jian Suo; Wen-Yu Han
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-21
Journal Detail:
Title:  Research in veterinary science     Volume:  -     ISSN:  1532-2661     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
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