| Identification and characterization of anonymous nuclear markers for the double-striped cockroach, Blattella bisignata. | |
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MedLine Citation:
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PMID: 22697889 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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During the last decade, multilocus analysis has gradually become a powerful tool for the studies of population genetics and phylogeography. The double-striped cockroach, Blattella bisignata, is endemic to southeast Asia, and there is currently little genetic information available for the species. We chose it as the target species to investigate a biodiversity hotspot in southwest China. Here, we report the identification and characterization of 11 single-copy anonymous nuclear markers with an average length of 378bp. These loci, isolated from a genomic library of B. bisignata, can amplify in two additional Blattella species (B. germanica and B. lituricollis). While testing these markers in representative species of Blattellidae, Blattidae and Epilampridae, some of them can cross-amplify successfully. After sequencing 30 individuals collected from southern China per locus, we found relatively high variability (approximately 3.6 SNPs per 100bp). Finally, a small-scale study was also performed to show that these markers do indeed fulfill the expectations as phylogeographic markers. |
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Authors:
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Q-P Ren; Z Fan; X-M Zhou; G-F Jiang; Y-T Wang; Y-X Liu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-15 |
Journal Detail:
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Title: Bulletin of entomological research Volume: - ISSN: 1475-2670 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984715R Medline TA: Bull Entomol Res Country: - |
Other Details:
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Languages: ENG Pagination: 1-7 Citation Subset: - |
Affiliation:
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Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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