| Identification and characterization of γ-aminobutyric acid uptake system GabPCg (NCgl0464) in Corynebacterium glutamicum. | |
| | |
MedLine Citation:
|
PMID: 22307305 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Corynebacterium glutamicum is widely used for industrial production of various amino acids and vitamins, and there is growing interest to engineering this bacterium for more commercial bioproducts such as γ-aminobutyric acid (GABA). In this study, a GABA specific transporter (GabP(Cg)) encoded by ncgl0464 was identified and characterized. GabP(Cg) plays a major role for GABA uptake and is essential to C. glutamicum growing on GABA. GABA uptake by GabP(Cg) was weakly competed by L-Asn and L-Gln and stimulated by sodium ion (Na(+)). The K(m) and V(max) values were determined to be 41.1 ± 4.5 μM and 36.8 ± 2.6 nmol min(-1)(mg DW)(-1), respectively, at pH of 6.5; and 34.2 ± 1.1 μM and 67.3 ± 1.0 nmol min(-1)(mg DW)(-1), respectively, at pH of 7.5. GabP(Cg) has 29% amino acid sequence identity to previously and functionally identified aromatic amino acid transporter (TyrP) of E. coli, but low identities to the currently known GABA transporters (17% and 15% to GabP(EC) and GabP(Bs), respectively). The GabP(Cg)-deleted mutant RES167Δncgl0464/pGXKZ29 showed 12.5% higher productivity of GABA compared to RES167/pGXKZ29. It is concluded that GabP(Cg) represents a new type of GABA transporters and is potentially important for engineering GABA-producing C. glutamicum strains. |
| | |
Authors:
|
Zhi Zhao; Jiu-Yuan Ding; Wen-Hua Ma; Ning-Yi Zhou; Shuang-Jiang Liu |
Related Documents
:
|
2925645 - A sulfated glucosylceramide from rat kidney. 22417505 - Utilization of african grains for sourdough bread making. 6610415 - Purification to apparent homogeneity of a thymocyte specific growth factor from calf th... 21612505 - Influence of a high cholesterol regime on epicardial and subcutaneous adipose tissue fa... 9175585 - Arachidonic acid inhibits 3h-glutamate uptake with different potencies in rodent centra... 1362315 - Glutamate and aspartate are decreased in the skin in amyotrophic lateral sclerosis. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-3 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-6 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
State Key Laboratory of Microbial Resources. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Differential biofilm formation and chemical disinfection resistance of sessile cells of Listeria mon...
Next Document: PATHOGENICITY AND INFECTION CYCLE OF VIBRIO OWENSII IN LARVICULTURE OF ORNATE SPINY LOBSTER (PANULIR...