Document Detail

Identification and characterization of ZFP-57, a novel zinc finger transcription factor in the mammalian peripheral nervous system.
MedLine Citation:
PMID:  15070898     Owner:  NLM     Status:  MEDLINE    
To isolate new zinc finger genes expressed at early stages of peripheral nerve development, we have used PCR to amplify conserved zinc finger sequences. RNA from rat embryonic day 12 and 13 sciatic nerves, a stage when nerves contain Schwann cell precursors, was used to identify several genes not previously described in Schwann cells. One of them, zinc finger protein (ZFP)-57, proved to be the homologue of a mouse gene found in F9 teratocarcinoma cells. Its mRNA expression profile within embryonic and adult normal and transected peripheral nerves, and its distribution in the rest of the nervous system is described. High levels of expression are seen in embryonic nerves and spinal cord. These drop rapidly during the first few weeks after birth, a pattern mirrored in other parts of the nervous system. ZFP-57 localizes to the nucleus of Schwann and other cells. The sequence contains an N-terminal Krüppel-associated box (KRAB) domain and ZFP-57 constructs containing green fluorescent protein reveal that the protein colocalizes with heterochromatin protein 1alpha to centromeric heterochromatin in a characteristic speckled pattern in NIH3T3 cells. The KRAB domain is required for this localization, because constructs lacking it target the protein to the nucleus but not to the centromeric heterochromatin. When fused to a heterologous DNA binding domain, the KRAB domain of ZFP-57 represses transcription, and full-length ZFP-57 represses Schwann cell transcription from myelin basic protein and P(0) promoters in co-transfection assays. Zfp-57 mRNA is up-regulated in Schwann cells in response to leukemia inhibitory factor and fibroblast growth factor 2.
María B Durán Alonso; Georg Zoidl; Carla Taveggia; Frank Bosse; Christiane Zoidl; Mary Rahman; Eric Parmantier; Charlotte H Dean; Brett S Harris; Lawrence Wrabetz; Hans Werner Müller; Kristjan R Jessen; Rhona Mirsky
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-04-07
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  279     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-07     Completed Date:  2004-07-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25653-64     Citation Subset:  IM    
Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom.
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MeSH Terms
Amino Acid Sequence
Cell Division
Cell Lineage
DNA-Binding Proteins / analysis*,  genetics,  physiology
Embryonic and Fetal Development
Fibroblast Growth Factor 2 / pharmacology
Interleukin-6 / pharmacology
Leukemia Inhibitory Factor
Molecular Sequence Data
Nuclear Proteins / analysis*,  genetics,  physiology
Promoter Regions, Genetic
RNA, Messenger / analysis
Repressor Proteins / physiology
Schwann Cells / chemistry*
Sciatic Nerve / chemistry*
Swiss 3T3 Cells
Zinc Fingers*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Interleukin-6; 0/Leukemia Inhibitory Factor; 0/Lif protein, mouse; 0/Nuclear Proteins; 0/RNA, Messenger; 0/Repressor Proteins; 0/Zfp-57 protein, rat; 103107-01-3/Fibroblast Growth Factor 2

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