Document Detail


Identification and characterization of an Arabidopsis homogentisate phytyltransferase paralog.
MedLine Citation:
PMID:  16408209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tocochromanols (tocopherols and tocotrienols) are micronutrients with antioxidant properties synthesized by photosynthetic bacteria and plants that play important roles in animal and human nutrition. There is considerable interest in identifying the genes involved in tocochromanol biosynthesis to allow transgenic modification of both tocochromanol levels and tocochromanol composition in agricultural crops. The first committed reaction in tocopherol biosynthesis is the condensation of homogentisic acid (HGA) with phytyldiphosphate or geranylgeranyldiphosphate, catalyzed by the homogentisate phytyltransferase (VTE2) or by the homogentisate geranylgeranyl transferase (HGGT). In this study, we describe the identification of conserved amino acid sequences within VTE2 and HGGT and the application of these conserved sequences for a motif analysis resulting in the discovery of a VTE2-paralog in the Arabidopsis genome. We designated this new gene VTE2-2 and renamed the old VTE2 to VTE2-1. Seed-specific expression of VTE2-2 in Arabidopsis resulted in increased seed-tocopherol levels, similar to the transgenic expression of VTE2-1. Bioinformatics analysis revealed that VTE2-2 is conserved in both monocotyledonous and dicotyledonous plants and is distinct from VTE2-1 and HGGT.
Authors:
Tyamagondlu V Venkatesh; Balasulojini Karunanandaa; Daniel L Free; Jeannie M Rottnek; Susan R Baszis; Henry E Valentin
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Publication Detail:
Type:  Journal Article     Date:  2006-01-12
Journal Detail:
Title:  Planta     Volume:  223     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-05     Completed Date:  2006-07-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1134-44     Citation Subset:  IM    
Affiliation:
Monsanto Company, 800 N. Lindbergh Boulevard, St. Louis, MO 63167, USA.
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MeSH Terms
Descriptor/Qualifier:
Alkyl and Aryl Transferases / chemistry,  genetics*,  metabolism
Amino Acid Motifs
Amino Acid Sequence
Antisense Elements (Genetics)
Arabidopsis / enzymology*,  genetics
Arabidopsis Proteins / chemistry,  genetics*,  metabolism
Computational Biology
Conserved Sequence
Molecular Sequence Data
Phylogeny
Plants, Genetically Modified / metabolism
Seeds / genetics,  metabolism
Sequence Alignment
Sequence Analysis, Protein
Tocopherols / metabolism*
Chemical
Reg. No./Substance:
0/Antisense Elements (Genetics); 0/Arabidopsis Proteins; 1406-66-2/Tocopherols; EC 2.5.-/Alkyl and Aryl Transferases; EC 2.5.-/HPT1 protein, Arabidopsis

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