Document Detail

Identification and characterization of microRNAs in raw milk during different periods of lactation, commercial fluid, and powdered milk products.
MedLine Citation:
PMID:  20548333     Owner:  NLM     Status:  MEDLINE    
Recent baby formula milk powder contamination incidents have shown that the classic markers or standards in milk quality control are insufficient in identifying "manipulated" poor-quality milk. In the present study, we demonstrated for the first time that cow milk contains large amounts of microRNAs (miRNAs) and that the unique expression profile of milk-specific miRNAs can serve as a novel indicator and possible new standard for the quality control of raw milk and milk-related commercial products, such as fluid milk and powdered formula milk. First, using Solexa sequencing, we systematically screened miRNA expression in raw milk and identified a total of 245 miRNAs in raw milk. Unlike other classic biomarkers whose expression levels are nearly identical at different periods of lactation, individual miRNAs can be significantly altered during lactation process, implicating that miRNAs may be a more accurate indicator to reflect the quality alteration of milk. Second, using TaqMan probe-based miRNA quantitative RT-PCR, we further identified seven miRNAs that have a relatively consistent expression throughout the lactation process, and more importantly, the expression profile of these seven milk-specific miRNAs can serve as an ideal biomarker for discriminating poor-quality or "manipulated" milk from pure raw milk, as well as for the quality control of commercial milk products, such as fluid milk and powdered formula milk. Together, our findings provide a basis for understanding the physiological role of milk miRNAs and a new potential standard for determining the quality of raw milk or milk-related commercial products.
Xi Chen; Chao Gao; Haijin Li; Lei Huang; Qi Sun; Yanye Dong; Chunliang Tian; Shengpu Gao; Hailin Dong; Danping Guan; Xiaoyun Hu; Shujian Zhao; Liang Li; Lin Zhu; Qiao Yan; Junfeng Zhang; Ke Zen; Chen-Yu Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-15
Journal Detail:
Title:  Cell research     Volume:  20     ISSN:  1748-7838     ISO Abbreviation:  Cell Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9425763     Medline TA:  Cell Res     Country:  China    
Other Details:
Languages:  eng     Pagination:  1128-37     Citation Subset:  IM    
Jiangsu Diabetes Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
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MeSH Terms
Biological Markers / analysis
Lactation / genetics*
MicroRNAs / analysis*,  metabolism
Milk / metabolism*,  standards
Quality Control
Reg. No./Substance:
0/Biological Markers; 0/MicroRNAs

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