Document Detail


Identification and characterisation of human pathogenic filamentous fungi and susceptibility to Thymus schimperi essential oil.
MedLine Citation:
PMID:  20633233     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Twenty-eight clinical fungal isolates were characterised by morphological (macro- and micro-features and growth response at 25, 30 and 37 °C) and molecular (nuclear rDNA-internal transcriber spacer, calmodulin, cytochrome c oxidase 1 and the largest subunit of RNA polymerase II) analyses. The clinical fungal isolates were ascribed to the following taxa: Penicillium chrysogenum, Verticillium sp., Aspergillus tubingensis, Aspergillus minutus, Beauveria bassiana and Microsporum gypseum. In addition, in vitro susceptibility testing of the isolates to conventional antifungal agents and to two chemically well-defined chemotypes of Thymus schimperi essential oil was performed. Most of the isolates were resistant to amphotericin B (except A. minutus), and itraconazole, while terbinafine was quite active on these fungi. T. schimperi essential oil showed antifungal activity against all of the tested fungal isolates with minimal inhibitory concentration values similar or lower than those of terbinafine. Transmission electron microscopy analyses revealed that fungal growth inhibition by essential oil was accompanied by marked morphological and cytological changes.
Authors:
Rita Pagiotti; Paola Angelini; Andrea Rubini; Bruno Tirillini; Bruno Granetti; Roberto Venanzoni
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Publication Detail:
Type:  Journal Article     Date:  2010-07-14
Journal Detail:
Title:  Mycoses     Volume:  54     ISSN:  1439-0507     ISO Abbreviation:  Mycoses     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8805008     Medline TA:  Mycoses     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  e364-76     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Verlag GmbH.
Affiliation:
Department of Applied Biology, University of Perugia, Perugia, Italy CNR - Institute of Plant Genetics, Perugia Division, Perugia, Italy Institute of Botany, University of Urbino, Urbino, Italy.
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