Document Detail


Identification and characterisation of an early/late bi-directional promoter of the capripoxvirus, lumpy skin disease virus.
MedLine Citation:
PMID:  10446656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Identification and characterisation of an early/late bi-directional promoter element of lumpy skin disease virus (LSDV) is described. The 56 bp element shows substantial structural similarities with other poxvirus promoters, providing further evidence that transcriptional elements are conserved within the Poxviridae. The relative strengths of the LSDV early and late promoters were compared to the vaccinia virus (VV) P7.5K and P11K promoters in transient expression assays. These transient assays also verified the temporally regulated nature of the promoter. Favourable expression levels suggest that the identified LSDV promoters could be used for expressing foreign genes in a recombinant LSDV system.
Authors:
W C Fick; G J Viljoen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of virology     Volume:  144     ISSN:  0304-8608     ISO Abbreviation:  Arch. Virol.     Publication Date:  1999  
Date Detail:
Created Date:  1999-09-02     Completed Date:  1999-09-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7506870     Medline TA:  Arch Virol     Country:  AUSTRIA    
Other Details:
Languages:  eng     Pagination:  1229-39     Citation Subset:  IM    
Affiliation:
Department of Genetics, University of Pretoria, South Africa.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M27624;  M35027;  U60315;  U95121;  X69198
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cattle
Genome, Viral*
Lumpy skin disease virus / genetics*
Molecular Sequence Data
Promoter Regions, Genetic*

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