Document Detail


Identification of cell wall proteins in the flax (Linum usitatissimum) stem.
MedLine Citation:
PMID:  23281244     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Sequential salt (CaCl(2) , LiCl) extractions were used to obtain fractions enriched in cell wall proteins from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR mass spectrometry analysis and the use of recently published genomic data allowed the identification of 11,912 peptides corresponding to a total of 1,418 different proteins. Subcellular localization using TargetP, Predotar and WoLF PSORT led to the identification of 152 putative flax cell wall proteins that were classified into 9 different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of cell wall proteins in flax and will lead to a better understanding of cell wall biology in this species.
Authors:
Arnaud Day; Stéphane Fénart; Godfrey Neutelings; Simon Hawkins; Christian Rolando; Caroline Tokarski
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-26
Journal Detail:
Title:  Proteomics     Volume:  -     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2013-1-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Université Lille Nord de France, Lille 1 UMR 1281, F-59650, Villeneuve d'Ascq cedex, France; INRA UMR 1281 Stress Abiotiques et Différenciation des Végétaux Cultivés, F-59650, Villeneuve d'Ascq, France.
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