Document Detail


Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover.
MedLine Citation:
PMID:  20828558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Assays to quantify myocardial renewal rely on the accurate identification of cardiomyocyte nuclei. We previously ¹⁴C birth dated human cardiomyocytes based on the nuclear localization of cTroponins T and I. A recent report by Kajstura et al. suggested that cTroponin I is only localized to the nucleus in a senescent subpopulation of cardiomyocytes, implying that ¹⁴C birth dating of cTroponin T and I positive cell populations underestimates cardiomyocyte renewal in humans. We show here that the isolation of cell nuclei from the heart by flow cytometry with antibodies against cardiac Troponins T and I, as well as pericentriolar material 1 (PCM-1), allows for isolation of close to all cardiomyocyte nuclei, based on ploidy and marker expression. We also present a reassessment of cardiomyocyte ploidy, which has important implications for the analysis of cell turnover, and iododeoxyuridine (IdU) incorporation data. These data provide the foundation for reliable analysis of cardiomyocyte turnover in humans.
Authors:
Olaf Bergmann; Sofia Zdunek; Kanar Alkass; Henrik Druid; Samuel Bernard; Jonas Frisén
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-07
Journal Detail:
Title:  Experimental cell research     Volume:  317     ISSN:  1090-2422     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-11-29     Completed Date:  2011-01-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  188-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Cell Nucleus / metabolism*
Cell Proliferation
Cell Separation
Flow Cytometry
Humans
Myocardium / metabolism
Myocytes, Cardiac / cytology,  metabolism,  ultrasonography*
Ploidies*
Troponin I / physiology
Troponin T / physiology
Chemical
Reg. No./Substance:
0/Troponin I; 0/Troponin T

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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