| Identification of cardiomyocyte nuclei and assessment of ploidy for the analysis of cell turnover. | |
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MedLine Citation:
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PMID: 20828558 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Assays to quantify myocardial renewal rely on the accurate identification of cardiomyocyte nuclei. We previously ¹⁴C birth dated human cardiomyocytes based on the nuclear localization of cTroponins T and I. A recent report by Kajstura et al. suggested that cTroponin I is only localized to the nucleus in a senescent subpopulation of cardiomyocytes, implying that ¹⁴C birth dating of cTroponin T and I positive cell populations underestimates cardiomyocyte renewal in humans. We show here that the isolation of cell nuclei from the heart by flow cytometry with antibodies against cardiac Troponins T and I, as well as pericentriolar material 1 (PCM-1), allows for isolation of close to all cardiomyocyte nuclei, based on ploidy and marker expression. We also present a reassessment of cardiomyocyte ploidy, which has important implications for the analysis of cell turnover, and iododeoxyuridine (IdU) incorporation data. These data provide the foundation for reliable analysis of cardiomyocyte turnover in humans. |
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Authors:
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Olaf Bergmann; Sofia Zdunek; Kanar Alkass; Henrik Druid; Samuel Bernard; Jonas Frisén |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-07 |
Journal Detail:
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Title: Experimental cell research Volume: 317 ISSN: 1090-2422 ISO Abbreviation: Exp. Cell Res. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 188-94 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Cell and Molecular Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Nucleus
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metabolism* Cell Proliferation Cell Separation Flow Cytometry Humans Myocardium / metabolism Myocytes, Cardiac / cytology, metabolism, ultrasonography* Ploidies* Troponin I / physiology Troponin T / physiology |
| Chemical | |
Reg. No./Substance:
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0/Troponin I; 0/Troponin T |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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