| Identification of candidate genes important for frost tolerance in Festuca pratensis Huds. by transcriptional profiling. | |
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MedLine Citation:
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PMID: 21421350 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Studies of differential gene expression between cold acclimated (CA) and non-cold acclimated (NA) plants yield insight into how plants prepare for cold stress at the transcriptional level. Furthermore genes involved in the cold acclimation process are good candidate loci for genetic variation in frost tolerance and winter survival. In this study we combine different approaches to try to decode the genetics of cold acclimation and frost tolerance in meadow fescue (Festuca pratensis Huds). An EST library of cold acclimation responsive genes was established by suppression subtractive hybridization (SSH), and a microarray experiment was used to identify gene expression differences between high and low frost tolerance genotypes in response to cold acclimation. Many genes known to be involved in CA in other species were confirmed to be involved in CA in F. pratensis, however, 18% of the ESTs did not show significant homology to any database proteins. Seven genes were found to be differentially expressed (>2-fold) between high and low frost tolerance genotypes. Two of these genes, FpQM and FpTPT, represent interesting candidate genes for frost tolerance in perennial forage grasses. |
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Authors:
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Heidi Rudi; Simen R Sandve; Lars M Opseth; Arild Larsen; Odd Arne Rognli |
Publication Detail:
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Type: Journal Article Date: 2010-07-24 |
Journal Detail:
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Title: Plant science (Shannon, Ireland) Volume: 180 ISSN: 0168-9452 ISO Abbreviation: Plant Sci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-03-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882015 Medline TA: Plant Sci Country: Ireland |
Other Details:
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Languages: eng Pagination: 78-85 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Norwegian University of Life Sciences, Department of Plant and Environmental Sciences, N-1432 Ås, Norway. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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