Document Detail


Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.
MedLine Citation:
PMID:  7565687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The c-mos proto-oncogene is specifically expressed in female and male germ cells. Previous studies identified a negative regulatory element (NRE) upstream of the c-mos promoter that suppresses c-mos transcription in transfected NIH 3T3 cells. In this study, we used gel shift assays to detect proteins in nuclear extracts of NIH 3T3 cells that bind to the c-mos NRE in a sequence-specific manner. One protein was found to bind to a region of the NRE which was shown by site-directed mutagenesis to be required for suppression of c-mos transcription. This factor was present in nuclear extracts of several somatic cell lines and tissues but not in male germ cells in which c-mos is transcribed, suggesting that it is a somatic cell repressor of c-mos transcription. The binding site of the candidate repressor within the c-mos NRE consists of sequences related to putative NREs identified in two other male germ cell-specific genes (encoding protamine 2 and phosphoglycerate kinase 2). The c-mos repressor bound and could be UV cross-linked to these protamine 2 and phosphoglycerate kinase 2 gene sequences as a protein with an apparent molecular mass of approximately 30 kDa. The repressor binding site is also conserved in two other germ cell-specific genes (encoding testis-specific cytochrome c and heat shock-like protein 70), suggesting that the c-mos repressor may be generally involved in suppressing transcription of germ cell-specific genes in somatic cells.
Authors:
W Xu; G M Cooper
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  15     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1995-10-25     Completed Date:  1995-10-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5369-75     Citation Subset:  IM    
Affiliation:
Division of Molecular Genetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Base Sequence
Cell Extracts
Cell Nucleus / metabolism
Consensus Sequence / genetics
Cytochrome c Group / genetics
DNA / metabolism
Female
Genes, mos / genetics*
HSP70 Heat-Shock Proteins / genetics
Male
Mice
Molecular Sequence Data
Molecular Weight
Phosphotransferases (Alcohol Group Acceptor) / genetics
Protamines / genetics
Regulatory Sequences, Nucleic Acid / genetics*
Repressor Proteins / chemistry,  metabolism*
Spermatozoa / metabolism*
Transcription, Genetic / genetics*
Grant Support
ID/Acronym/Agency:
R01 HD26594/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Cell Extracts; 0/Cytochrome c Group; 0/HSP70 Heat-Shock Proteins; 0/Protamines; 0/Repressor Proteins; 0/protamine 2; 9007-49-2/DNA; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.31/glycerate kinase
Comments/Corrections

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