| Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes. | |
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MedLine Citation:
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PMID: 7565687 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The c-mos proto-oncogene is specifically expressed in female and male germ cells. Previous studies identified a negative regulatory element (NRE) upstream of the c-mos promoter that suppresses c-mos transcription in transfected NIH 3T3 cells. In this study, we used gel shift assays to detect proteins in nuclear extracts of NIH 3T3 cells that bind to the c-mos NRE in a sequence-specific manner. One protein was found to bind to a region of the NRE which was shown by site-directed mutagenesis to be required for suppression of c-mos transcription. This factor was present in nuclear extracts of several somatic cell lines and tissues but not in male germ cells in which c-mos is transcribed, suggesting that it is a somatic cell repressor of c-mos transcription. The binding site of the candidate repressor within the c-mos NRE consists of sequences related to putative NREs identified in two other male germ cell-specific genes (encoding protamine 2 and phosphoglycerate kinase 2). The c-mos repressor bound and could be UV cross-linked to these protamine 2 and phosphoglycerate kinase 2 gene sequences as a protein with an apparent molecular mass of approximately 30 kDa. The repressor binding site is also conserved in two other germ cell-specific genes (encoding testis-specific cytochrome c and heat shock-like protein 70), suggesting that the c-mos repressor may be generally involved in suppressing transcription of germ cell-specific genes in somatic cells. |
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Authors:
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W Xu; G M Cooper |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular and cellular biology Volume: 15 ISSN: 0270-7306 ISO Abbreviation: Mol. Cell. Biol. Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1995-10-25 Completed Date: 1995-10-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 8109087 Medline TA: Mol Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5369-75 Citation Subset: IM |
Affiliation:
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Division of Molecular Genetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Base Sequence Cell Extracts Cell Nucleus / metabolism Consensus Sequence / genetics Cytochrome c Group / genetics DNA / metabolism Female Genes, mos / genetics* HSP70 Heat-Shock Proteins / genetics Male Mice Molecular Sequence Data Molecular Weight Phosphotransferases (Alcohol Group Acceptor) / genetics Protamines / genetics Regulatory Sequences, Nucleic Acid / genetics* Repressor Proteins / chemistry, metabolism* Spermatozoa / metabolism* Transcription, Genetic / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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R01 HD26594/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Cytochrome c Group; 0/HSP70 Heat-Shock Proteins; 0/Protamines; 0/Repressor Proteins; 0/protamine 2; 9007-49-2/DNA; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.31/glycerate kinase |
| Comments/Corrections | |
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