Document Detail


Identification of a c-myb attenuator-binding factor.
MedLine Citation:
PMID:  11755468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression of the c-myb proto-oncogene is developmentally regulated at the level of transcription elongation. In pre-B cells, complete c-myb transcripts are produced, whereas transcripts are attenuated near or within a 300-base pair (bp) interval of the first c-myb intron in mature cells. Hypothesizing that transcription attenuation results from a protein complex that physically impedes the progress of RNA polymerase II through the intron, we used electrophoretic mobility shift assays (EMSA) to search for DNA-binding activities that correlated with downregulation of c-myb transcription. We identified a stage-specific DNA binding activity, termed ABF, present in mature B cells but not in pre-B cells. ABF binds to a 15-bp DNA element located within a 300-bp BstEII-XbaI fragment. DMSO-treatment of murine erythroleukemia cells results in rapid downregulation of c-myb transcription and upregulation of ABF DNA binding activity. Thus, ABF binding activity correlates with downregulation of c-myb transcription in two systems. Preliminary biochemical characterization of ABF from mature B cells demonstrates that its primary DNA-binding component is a 64-kDa-protein. We hypothesize that this factor may represent a member of the transcriptional attenuation complex.
Authors:
Jeffrey M Perkel; M Celeste Simon; Anjana Rao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Leukemia research     Volume:  26     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2001-12-28     Completed Date:  2002-03-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  179-90     Citation Subset:  IM    
Affiliation:
Abramson Family Cancer Research Institute, University of Pennsylvania, Room 456, BRB 11/111, 421 Curie Boulevard, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocytes / metabolism*
Blotting, Northern
DNA / genetics,  metabolism*
DNA-Binding Proteins / genetics,  isolation & purification*,  metabolism,  physiology
Dimethyl Sulfoxide / pharmacology
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Developmental
Gene Expression Regulation, Leukemic
Genes, myb*
Introns
Leukemia, Erythroblastic, Acute / metabolism
Lipopolysaccharides / pharmacology
Mice
Molecular Weight
NF-kappa B / metabolism
Neoplasm Proteins / biosynthesis,  genetics
Operon*
Proto-Oncogene Proteins c-myb / biosynthesis,  genetics
RNA Polymerase II / metabolism
Recombinant Fusion Proteins
Transcription, Genetic
Transfection
Tumor Cells, Cultured / drug effects,  metabolism
Grant Support
ID/Acronym/Agency:
CA42471/CA/NCI NIH HHS; GM19556-01A1/GM/NIGMS NIH HHS; HL52094/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Lipopolysaccharides; 0/NF-kappa B; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-myb; 0/Recombinant Fusion Proteins; 0/c-myb attenuator-binding factor; 67-68-5/Dimethyl Sulfoxide; 9007-49-2/DNA; EC 2.7.7.-/RNA Polymerase II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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