Document Detail


Identification of c-kit gene mutations in primary adenoid cystic carcinoma of the salivary gland.
MedLine Citation:
PMID:  19617878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The CD117 (KIT) protein is overexpressed in many human neoplasms including adenoid cystic carcinoma of salivary glands. To evaluate the function of c-kit-activating mutations in adenoid cystic carcinoma of the salivary gland, we studied 14 cases (13 primary, 1 cervical lymph node metastasis) from our institution. KIT protein expression was evaluated by immunohistochemistry using formalin-fixed paraffin-embedded tissue. Mutational analyses of c-kit extracellular (exon 9), juxtamembrane (exon 11) and tyrosine kinase domains (exons 13 and 17) were performed by polymerase chain reaction, clonal selection and DNA sequencing. All 14 cases demonstrated strong KIT expression by immunohistochemistry. Molecular analysis was successful in 8 of 14 cases, and c-kit missense point mutations were detected in seven of eight cases (88%) including seven in exon 11, two in exon 9, two in exon 13 and two in exon 17. Eight silent point mutations were detected in five cases. Two cases contained missense mutations in more than one exon. Different mutations were found in the primary tumor and the cervical lymph node metastasis of one patient. Point mutations in domains similar to those described in gastrointestinal stromal tumors were detected, including Pro551Leu and Lys558Glu (5' end of exon 11), Leu576Phe (3' end of exon 11), Val643Ala (exon 13) and Asn822Ser (exon 17). Additional novel point mutations in exons 9, 11, 13 and 17 were also identified. This study is the first to report c-kit gene mutations in primary adenoid cystic carcinoma of the salivary gland. Identification of such potential gain-of-function mutations in exon 11, and less frequently in exons 9, 13 and 17, suggests that KIT may be involved in the pathogenesis of adenoid cystic carcinoma of salivary glands. Our study raises a prospect of correlation of c-kit mutation and a potential treatment of adenoid cystic carcinoma with tyrosine kinase inhibitor (imatinib).
Authors:
Lizette Vila; Hongyan Liu; Samer Z Al-Quran; Dominique P Coco; Hui-Jia Dong; Chen Liu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-07-17
Journal Detail:
Title:  Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc     Volume:  22     ISSN:  1530-0285     ISO Abbreviation:  Mod. Pathol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-30     Completed Date:  2009-12-03     Revised Date:  2013-08-28    
Medline Journal Info:
Nlm Unique ID:  8806605     Medline TA:  Mod Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1296-302     Citation Subset:  IM    
Affiliation:
Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Carcinoma, Adenoid Cystic / chemistry,  genetics*,  secondary
DNA Mutational Analysis
Exons
Female
Gene Expression Regulation, Neoplastic*
Humans
Immunohistochemistry
Lymphatic Metastasis
Male
Middle Aged
Mutation, Missense*
Point Mutation*
Polymerase Chain Reaction
Proto-Oncogene Proteins c-kit / analysis,  genetics*
Salivary Gland Neoplasms / chemistry,  genetics*,  secondary
Grant Support
ID/Acronym/Agency:
DK02958/DK/NIDDK NIH HHS; K08 DK002958/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
EC 2.7.10.1/Proto-Oncogene Proteins c-kit
Comments/Corrections
Comment In:
Mod Pathol. 2010 Jun;23(6):905-6; author reply 906-7   [PMID:  20514080 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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