Document Detail

Identification of bistable populations of Porphyromonas gingivalis that differ in epithelial cell invasion.
MedLine Citation:
PMID:  20576685     Owner:  NLM     Status:  MEDLINE    
Bistable populations of bacteria give rise to two or more subtypes that exhibit different phenotypes. We have explored whether the periodontal pathogen Porphyromonas gingivalis exhibits bistable invasive phenotypes. Using a modified cell invasion assay, we show for the first time that there are two distinct subtypes within a population of P. gingivalis strains NCTC 11834 and W50 that display differences in their ability to invade oral epithelial cells. The highly invasive subtype invades cells at 10-30-fold higher levels than the poorly invasive subtype and remains highly invasive for approximately 12-16 generations. Analysis of the gingipain activity of these subtypes revealed that the highly invasive type had reduced cell-associated arginine-specific protease activity. The role of Arg-gingipain activity in invasion was verified by enhancement of invasion by rgpAB mutations and by inclusion of an Arg-gingipain inhibitor in invasion assays using wild-type bacteria. In addition, a population of ΔrgpAB bacteria did not contain a hyperinvasive subtype. Screening of the protease activity of wild-type populations of both strains identified high and low protease subtypes which also showed a corresponding reduction or enhancement, respectively, of invasive capabilities. Microarray analysis of these bistable populations revealed a putative signature set of genes that includes oxidative stress resistance and iron transport genes, and which might be critical to invasion of or survival within epithelial cells.
S Suwannakul; G P Stafford; S A Whawell; C W I Douglas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-24
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  156     ISSN:  1465-2080     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2010-12-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  3052-64     Citation Subset:  IM    
Oral and Maxillofacial Pathology, School of Clinical Dentistry, The University of Sheffield, Sheffield S10 2TA, UK.
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MeSH Terms
Adhesins, Bacterial / metabolism*
Bacteroidaceae Infections / microbiology*
Cell Line
Cysteine Endopeptidases / metabolism*
Epithelial Cells / microbiology*
Gene Expression Profiling
Hydrogen Peroxide / metabolism
Oligonucleotide Array Sequence Analysis
Oxidative Stress
Peptide Hydrolases / metabolism
Porphyromonas gingivalis / enzymology,  genetics,  pathogenicity*
Reg. No./Substance:
0/Adhesins, Bacterial; 7722-84-1/Hydrogen Peroxide; EC 3.4.-/Peptide Hydrolases; EC 3.4.22.-/Cysteine Endopeptidases; EC, Porphyromonas gingivalis

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