| Identification of biochemical changes in lactovegetarian urine using 1H NMR spectroscopy and pattern recognition. | |
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MedLine Citation:
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PMID: 20016880 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A vegetarian diet has been demonstrated to have a profound influence on human metabolism as well as to aid the prevention of several chronic diseases relative to an omnivorous diet. However, there have been no systematic metabolomic studies on all of the biochemical changes induced in human subjects by long-term vegetarianism. In this study, (1)H NMR spectroscopy in combination with multivariate statistical analysis was applied to explore the variability in the metabolic urinary profiles of healthy populations from four groups: lactovegetarian male (VEGMALE), lactovegetarian female (VEGFEMALE), omnivorous male (OMNMALE), and omnivorous female (OMNFEMALE). Differences in metabolic profiles were examined in relation to diet and gender by principal component analysis (PCA) and spectral integrals. It was found that the most influential low molecular weight metabolites responsible for the differences between the diet groups were N-acetyl glycoprotein (NAG), succinate, citrate, trimethylamine-N-oxide (TMAO), taurine, glycine, hippurate, phenylalanine, methylhistidine and formate, whereas for the differences in gender groups the most discriminatory metabolites were NAG, succinate, creatinine, arginine, TMAO, taurine, hippurate, mannitol, phenylalanine, and methylhistidine. The results from the PCA of all four groups indicated that diet plays a greater role in influencing metabolite differences than gender. As an exploration, this work shows the potential of metabolomics when applied to nutritional and physiological studies, and it will aid further studies. |
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Authors:
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Jingjing Xu; Shuyu Yang; Shuhui Cai; Jiyang Dong; Xuejun Li; Zhong Chen |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-12-17 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 396 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1451-63 Citation Subset: IM |
Affiliation:
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Department of Physics, Fujian Key Laboratory of Plasma and Magnetic Resonance, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen 361005, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Diet, Vegetarian* Female Glucose / analysis Glycine / urine Glycosuria Humans Magnetic Resonance Spectroscopy Male Metabolomics Middle Aged Multivariate Analysis Questionnaires Reference Values Sex Factors Succinic Acid / urine Taurine / urine Urine / chemistry* Young Adult |
| Chemical | |
Reg. No./Substance:
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107-35-7/Taurine; 110-15-6/Succinic Acid; 50-99-7/Glucose; 56-40-6/Glycine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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