| Identification of the bicarbonate secretory cell of the turtle bladder. | |
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MedLine Citation:
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PMID: 4073269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The turtle bladder consists primarily of two mucosal cell types, mitochondrial rich (MR) and granular (G) cells. The MR cells secrete H+. In addition, the bladder secretes HCO3-, an energy-requiring Cl(-)-dependent transport system. These studies were designed to identify the cell type responsible for HCO3- secretion. Specific HCO3- and H+ secretory inhibitors were added to alter O2 consumption of MR and G cells. Cells were separated by Ficoll density centrifugation. Ouabain (10(-4) M) was used in all studies to eliminate O2 consumption associated with Na+ transport. O2 consumption of cells treated with 10(-4) M 4-acetamido-4'-isothiocyanostilbene-2,2'-sulfonic acid (SITS), which indirectly inhibits H+ secretion, was compared with cells additionally treated with 5 X 10(-4) M acetazolamide, an inhibitor of both H+ and HCO3- secretion. O2 consumption of MR cells treated with SITS alone was significantly greater than that of MR cells additionally treated with acetazolamide (delta = 0.56 +/- 0.10 microliter O2 X h-1 X mg protein-1, n = 6, P less than 0.003). There was no significant difference in G cells similarly treated. Another means of selectively altering H+ and HCO3- transports and their associated metabolic rates is to manipulate the Cl- concentration of the incubation medium. In a Cl(-)-rich medium, HCO3- and H+ transports are maximized. In low Cl(-)-SO2-4 medium, HCO3- secretion is selectively reduced.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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C Fritsche; J H Schwartz |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 249 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1985 Dec |
Date Detail:
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Created Date: 1986-01-23 Completed Date: 1986-01-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F858-62 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
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pharmacology Acetazolamide / pharmacology Animals Bicarbonates / secretion* Chlorides / metabolism Hydrogen / metabolism Ouabain / pharmacology Oxygen Consumption / drug effects Turtles / anatomy & histology* Urinary Bladder / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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AM-20611/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bicarbonates; 0/Chlorides; 1333-74-0/Hydrogen; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 59-66-5/Acetazolamide; 630-60-4/Ouabain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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