Document Detail

Identification of the bicarbonate secretory cell of the turtle bladder.
MedLine Citation:
PMID:  4073269     Owner:  NLM     Status:  MEDLINE    
The turtle bladder consists primarily of two mucosal cell types, mitochondrial rich (MR) and granular (G) cells. The MR cells secrete H+. In addition, the bladder secretes HCO3-, an energy-requiring Cl(-)-dependent transport system. These studies were designed to identify the cell type responsible for HCO3- secretion. Specific HCO3- and H+ secretory inhibitors were added to alter O2 consumption of MR and G cells. Cells were separated by Ficoll density centrifugation. Ouabain (10(-4) M) was used in all studies to eliminate O2 consumption associated with Na+ transport. O2 consumption of cells treated with 10(-4) M 4-acetamido-4'-isothiocyanostilbene-2,2'-sulfonic acid (SITS), which indirectly inhibits H+ secretion, was compared with cells additionally treated with 5 X 10(-4) M acetazolamide, an inhibitor of both H+ and HCO3- secretion. O2 consumption of MR cells treated with SITS alone was significantly greater than that of MR cells additionally treated with acetazolamide (delta = 0.56 +/- 0.10 microliter O2 X h-1 X mg protein-1, n = 6, P less than 0.003). There was no significant difference in G cells similarly treated. Another means of selectively altering H+ and HCO3- transports and their associated metabolic rates is to manipulate the Cl- concentration of the incubation medium. In a Cl(-)-rich medium, HCO3- and H+ transports are maximized. In low Cl(-)-SO2-4 medium, HCO3- secretion is selectively reduced.(ABSTRACT TRUNCATED AT 250 WORDS)
C Fritsche; J H Schwartz
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  249     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-23     Completed Date:  1986-01-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F858-62     Citation Subset:  IM    
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MeSH Terms
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / pharmacology
Acetazolamide / pharmacology
Bicarbonates / secretion*
Chlorides / metabolism
Hydrogen / metabolism
Ouabain / pharmacology
Oxygen Consumption / drug effects
Turtles / anatomy & histology*
Urinary Bladder / cytology*
Grant Support
Reg. No./Substance:
0/Bicarbonates; 0/Chlorides; 1333-74-0/Hydrogen; 27816-59-7/4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 59-66-5/Acetazolamide; 630-60-4/Ouabain

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