Document Detail


Identification of betalains from petioles of differently colored Swiss chard (Beta vulgaris L. ssp. cicla [L.] Alef. Cv. Bright Lights) by high-performance liquid chromatography-electrospray ionization mass spectrometry.
MedLine Citation:
PMID:  15137842     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The betalain pattern of differently colored Swiss chard (Beta vulgaris L. ssp. cicla [L.] Alef. cv. Bright Lights) was investigated for the first time. Nineteen betaxanthins and nine betacyanins were identified by RP-HPLC and positive ion electrospray mass spectrometry, co-injection experiments with semisynthetic reference compounds, and standards derived from authentic plant material, respectively. Histamine-betaxanthin and alanine-betaxanthin were found to be novel betaxanthins, which to the best of our knowledge have not been reported as natural compounds until now. Furthermore, tyramine-betaxanthin (miraxanthin III) and 3-methoxytyramine-betaxanthin, which to date were known only from families other than the Chenopodiaceae, were detected for the first time in colored Swiss chard. The betacyanin pattern of purple petioles was composed of betanin, isobetanin, betanidin, and isobetanidin. Although phyllocactin was present in only trace amounts, further acylated structures such as betanidin-monoferuloyl-5-O-beta-diglucoside and lampranthin II, accompanied by their corresponding C(15)-epimers, were identified. In addition, quantification of betalains and CIE LCh degrees measurements were performed with the colored extracts to correlate the visual appearance with the respective pigment patterns. Besides the novel phytochemical findings, the present study is useful for the evaluation of betalainic Swiss chard as a potential coloring foodstuff.
Authors:
Florian Kugler; Florian C Stintzing; Reinhold Carle
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  52     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2004 May 
Date Detail:
Created Date:  2004-05-12     Completed Date:  2004-07-07     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2975-81     Citation Subset:  IM    
Affiliation:
Institute of Food Technology, Section Plant Foodstuff Technology, Hohenheim University, August-von-Hartmann-Strasse 3, D-70599 Stuttgart, Germany.
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MeSH Terms
Descriptor/Qualifier:
Beta vulgaris / chemistry*
Betalains
Chromatography, High Pressure Liquid*
Food Coloring Agents
Pigments, Biological / chemistry*
Plant Leaves / chemistry
Quaternary Ammonium Compounds / analysis*
Spectrometry, Mass, Electrospray Ionization*
Chemical
Reg. No./Substance:
0/Food Coloring Agents; 0/Pigments, Biological; 0/Quaternary Ammonium Compounds; 37279-84-8/Betalains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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