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Identification of benzo[a]pyrene-induced cell cycle-associated alterations in MCF-7 cells using infrared spectroscopy with computational analysis.
MedLine Citation:
PMID:  22561278     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chemical contaminants, such as benzo[a]pyrene (B[a]P), may modulate transcriptional responses in cells via the activation of aryl hydrocarbon receptor (AhR) or through responses to DNA damage following adduct formation. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy can be employed in a non-destructive fashion to interrogate the biochemical signature of cells via generation of infrared (IR) spectra. By applying to generated spectral datasets subsequent computational approaches such as principal component analysis plus linear discriminant analysis (PCA-LDA), derived data reduction is achieved to facilitate the visualization of wavenumber-related alterations in target cells. Discriminating spectral variables might be associated with lipid or glycogen content, conformational protein changes and phosphorylation, and structural alterations in DNA/RNA. Using this approach, we investigated the dose-related effects of B[a]P in MCF-7 cells concentrated in S- or G(0)/G(1)-phase. Our findings identified that in PCA-LDA scores plots a clear segregation of IR spectra was evident, with the major spectral alterations associated with DNA/RNA, secondary protein structure and lipid. Dose-related effects were observed and even with exposures as low as 10(-9)M B[a]P, significant (P≤0.001) separation of B[a]P-treated vs. vehicle control cells was noted. ATR-FTIR spectroscopy and computational approaches are a novel approach to identify the effects of environmental contaminants in target cells.
Authors:
Weiyi Pang; Junyi Li; Abdullah A Ahmadzai; Lara Heppenstall; Valon Llabjani; Júlio Trevisan; Xiaoqiang Qiu; Francis L Martin
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-26
Journal Detail:
Title:  Toxicology     Volume:  -     ISSN:  1879-3185     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-5-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0361055     Medline TA:  Toxicology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ireland Ltd.
Affiliation:
The School of Public Health, Guangxi Medical University, Nanning, Guangxi 530021, PR China; The Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, United Kingdom.
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