Document Detail

Identification of autotaxin as a neurite retraction-inducing factor of PC12 cells in cerebrospinal fluid and its possible sources.
MedLine Citation:
PMID:  15686493     Owner:  NLM     Status:  MEDLINE    
Abstract Cerebrospinal fluid (CSF) induced neurite retraction of differentiated PC12 cells; the action was observed in 15 min (a rapid response) and the activity further increased until 6 h (a long-acting response) during exposure of CSF to the cells. The CSF action was sensitive to monoglyceride lipase and diminished by homologous desensitization with lysophosphatidic acid (LPA) and by pretreatment with an LPA receptor antagonist Ki16425. Although fresh CSF contains LPA to some extent, the LPA content in the medium was increased during culture of PC12 cells with CSF. The rapid response was mimicked by exogenous LPA, and a long-acting response was duplicated by a recombinant autotaxin, lysophospholipase D (lyso-PLD). Although the lyso-PLD substrate lysophosphatidylcholine (LPC) was not detected in CSF, lyso-PLD activity and an approximately 120-kDa autotaxin protein were detected in CSF. On the other hand, LPC but not lyso-PLD activity was detected in the conditioned medium of a PC12 cell culture without CSF. Among neural cells examined, leptomeningeal cells expressed the highest lyso-PLD activity and autotaxin protein. These results suggest that leptomeningeal cells may work as one of the sources for autotaxin, which may play a critical role in LPA production and thereby regulate axonal and neurite morphological change.
Koichi Sato; Enkhzol Malchinkhuu; Takeshi Muraki; Koichi Ishikawa; Kensuke Hayashi; Masahiko Tosaka; Akikazu Mochiduki; Kinji Inoue; Hideaki Tomura; Chihiro Mogi; Hiromi Nochi; Koichi Tamoto; Fumikazu Okajima
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  92     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-02-02     Completed Date:  2005-03-21     Revised Date:  2011-11-07    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  904-14     Citation Subset:  IM    
Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.
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MeSH Terms
Autocrine Motility Factor / cerebrospinal fluid*,  physiology*
Cells, Cultured
Glycoproteins / cerebrospinal fluid*,  physiology*
Isoxazoles / pharmacology
Lysophospholipids / pharmacology
Multienzyme Complexes / cerebrospinal fluid*,  physiology*
Neurites / chemistry,  drug effects,  metabolism*
PC12 Cells
Phosphodiesterase I
Propionic Acids / pharmacology
Rats, Wistar
Receptors, Lysophosphatidic Acid / antagonists & inhibitors,  metabolism
Reg. No./Substance:
0/3-(4-(4-((1-(2-chlorophenyl)ethoxy)carbonyl amino)-3-methyl-5-isoxazolyl) benzylsulfanyl) propanoic acid; 0/Autocrine Motility Factor; 0/Glycoproteins; 0/Isoxazoles; 0/Lysophospholipids; 0/Multienzyme Complexes; 0/Propionic Acids; 0/Receptors, Lysophosphatidic Acid; 22002-87-5/lysophosphatidic acid; EC I; EC phosphodiesterase; EC 3.6.1.-/Pyrophosphatases

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