| Identification of autoantibody biomarkers for primary Sjögren's syndrome using protein microarrays. | |
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MedLine Citation:
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PMID: 21413148 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sjögren’s syndrome (SS) is a chronic, progressive autoimmune disease primarily affecting women. Diagnosis of SS requires an invasive salivary gland tissue biopsy and a long delay from the start of the symptoms to final diagnosis has been frequently observed. In this study,we aim to identify salivary autoantibody biomarkers for primary SS (pSS) using a protein microarray approach. Immune-response protoarrays were used to profile saliva autoantibodies from patients with pSS (n = 514), patients with systemic lupus erythematosus(SLE, n = 513), and healthy control subjects (n = 513). We identified 24 potential autoantibody biomarkers that can discriminate patients with pSS from both patients with SLE and healthy individuals. Four saliva autoantibody biomarkers, anti-transglutaminase, anti-histone, anti-SSA, and anti-SSB, were further tested in independent pSS (n = 534), SLE (n = 534), and healthy control (n = 534) subjects and all were successfully validated with ELISA. This study has demonstrated the potential of a high-throughput protein microarray approach for the discovery of autoantibody biomarkers. The identified saliva autoantibody biomarkers may lead to a clinical tool for simple, noninvasive detection of pSS at low cost. |
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Authors:
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Shen Hu; Arjan Vissink; Martha Arellano; Caroline Roozendaal; Hui Zhou; Cees G M Kallenberg; David T Wong |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-03-17 |
Journal Detail:
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Title: Proteomics Volume: 11 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-04-07 Completed Date: 2011-09-22 Revised Date: 2011-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 1499-507 Citation Subset: IM |
Affiliation:
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School of Dentistry, University of California, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA. shenhu@ucla.edu. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Antinuclear
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analysis Autoantibodies / analysis*, immunology Biological Markers / analysis Enzyme-Linked Immunosorbent Assay Histones / immunology Humans Lupus Erythematosus, Systemic / immunology Protein Array Analysis Saliva / immunology Sjogren-Larsson Syndrome / diagnosis, immunology*, metabolism Transglutaminases / immunology |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE017593-02/DE/NIDCR NIH HHS; R01DE017593/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Antinuclear; 0/Autoantibodies; 0/Biological Markers; 0/Histones; 0/SS-A antibodies; 0/SS-B antibodies; EC 2.3.2.13/Transglutaminases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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