| Identification of atypical ether-linked glycerophospholipid species in macrophages by mass spectrometry. | |
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MedLine Citation:
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PMID: 19965583 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A large scale profiling and analysis of glycerophospholipid species in macrophages has facilitated the identification of several rare and atypical glycerophospholipid species. By using liquid chromatography tandem mass spectrometry and comparison of the elution and fragmentation properties of the rare lipids to synthetic standards, we were able to identify an array of ether-linked phosphatidylinositols (PIs), phosphatidic acids, phosphatidylserines (PSs), very long chain phosphatidylethanolamines (PEs), and phosphatidylcholines (PCs) as well as phosphatidylthreonines (PTs) and a wide collection of odd carbon fatty acid-containing phospholipids in macrophages. A comprehensive qualitative analysis of glycerophospholipids from different macrophage cells was conducted. During the phospholipid profiling of the macrophage-like RAW 264.7 cells, we identified dozens of rare or previously uncharacterized phospholipids, including ether-linked PIs, PSs, and glycerophosphatidic acids, PTs, and PCs and PTs containing very long polyunsaturated fatty acids. Additionally, large numbers of phospholipids containing at least one odd carbon fatty acid were identified. Using the same methodology, we also identified many of the same species of glycerophospholipids in resident peritoneal macrophages, foam cells, and murine bone marrow derived macrophages. |
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Authors:
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Pavlina T Ivanova; Stephen B Milne; H Alex Brown |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-11-30 |
Journal Detail:
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Title: Journal of lipid research Volume: 51 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-12 Completed Date: 2010-08-17 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 1581-90 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Chemistry, Vanderbilt University Medical Center, Nashville, TN 37232, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Ether, Ethyl / chemistry* Fatty Acids / chemistry Female Glycerophospholipids / analysis*, chemistry* Macrophages / chemistry* Mass Spectrometry* Mice |
| Grant Support | |
ID/Acronym/Agency:
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U54GM69338/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Glycerophospholipids; 60-29-7/Ether, Ethyl |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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