Document Detail


Identification of ancillary fim genes affecting fimA expression in Salmonella typhimurium.
MedLine Citation:
PMID:  1360005     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regulation of the gene, fimA, encoding the major fimbrial subunit of S. typhimurium S6704 was examined by using a lambda fimA-lacZ lysogen. Transformation of the lambda fimA-lacZ lysogen with various derivatives of the recombinant plasmid that encodes type 1 fimbrial expression, pISF101, indicated that two regions of this plasmid alter beta-galactosidase production. One plasmid is a deletion resulting in the loss of a 28-kDa polypeptide downstream of fimA, while the other plasmid encodes a 24- and a 27-kDa polypeptide. Northern (RNA) blot analyses indicated that the steady-state fimA mRNA levels of these transformants were high. In addition, phenotypic expression of type 1 fimbriae by agar-grown cultures is observed only in those transformants bearing plasmids which show increased beta-galactosidase and fimA mRNA levels.
Authors:
D L Swenson; S Clegg
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  174     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1992-12-30     Completed Date:  1992-12-30     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7697-704     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/L01135;  L01136;  L01137;  L01138;  L01139;  L01140;  L01141;  M90677;  M98391;  Z11768
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Bacterial Proteins / biosynthesis,  genetics*
Base Sequence
DNA Mutational Analysis
DNA-Binding Proteins
Fimbriae Proteins*
Fimbriae, Bacterial / physiology*
Gene Expression Regulation, Bacterial*
Genes, Bacterial / genetics*
Membrane Proteins
Molecular Sequence Data
RNA, Messenger / biosynthesis
Recombinant Fusion Proteins
Salmonella typhimurium / genetics*
Transformation, Genetic
beta-Galactosidase / biosynthesis,  genetics
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Membrane Proteins; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/fimbrillin; 147680-16-8/Fimbriae Proteins; EC 3.2.1.23/beta-Galactosidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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