Document Detail


Identification and analysis of new proteins involved in the DNA damage response network of Fanconi anemia and Bloom syndrome.
MedLine Citation:
PMID:  19245838     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of co-immunoprecipitation (co-IP) to purify multi-protein complexes has contributed greatly to our understanding of the DNA damage response network associated with Fanconi anemia (FA), Bloom syndrome (BS) and breast cancer. Four new FA genes and two new protein partners for the Bloom syndrome gene product have been identified by co-IP. Here, we discuss our experience in using co-IP and other techniques to isolate and characterize new FA and BS-related proteins.
Authors:
Rong Guo; Dongyi Xu; Weidong Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2009-02-24
Journal Detail:
Title:  Methods (San Diego, Calif.)     Volume:  48     ISSN:  1095-9130     ISO Abbreviation:  Methods     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-05-27     Completed Date:  2009-08-13     Revised Date:  2013-04-24    
Medline Journal Info:
Nlm Unique ID:  9426302     Medline TA:  Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  72-9     Citation Subset:  IM    
Affiliation:
Laboratory of Genetics, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, Baltimore, MD 21224, USA.
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MeSH Terms
Descriptor/Qualifier:
Bloom Syndrome / genetics*,  metabolism
Carrier Proteins / analysis*,  isolation & purification,  metabolism
DNA Damage*
DNA Topoisomerases, Type I / analysis*,  isolation & purification,  metabolism
DNA-Binding Proteins / analysis*,  isolation & purification,  metabolism
Fanconi Anemia / genetics*,  metabolism
Humans
Nuclear Proteins / analysis*,  isolation & purification,  metabolism
RecQ Helicases / analysis*,  isolation & purification,  metabolism
Grant Support
ID/Acronym/Agency:
Z01 AG000657-08/AG/NIA NIH HHS; Z01 AG000657-08/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/RMI1 protein, human; 0/RMI2 protein, human; EC 3.6.1.-/Bloom syndrome protein; EC 3.6.1.-/RecQ Helicases; EC 5.99.1.2/DNA Topoisomerases, Type I; EC 5.99.1.2/DNA topoisomerase III
Comments/Corrections

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