| Identification and analysis of new proteins involved in the DNA damage response network of Fanconi anemia and Bloom syndrome. | |
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MedLine Citation:
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PMID: 19245838 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The use of co-immunoprecipitation (co-IP) to purify multi-protein complexes has contributed greatly to our understanding of the DNA damage response network associated with Fanconi anemia (FA), Bloom syndrome (BS) and breast cancer. Four new FA genes and two new protein partners for the Bloom syndrome gene product have been identified by co-IP. Here, we discuss our experience in using co-IP and other techniques to isolate and characterize new FA and BS-related proteins. |
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Authors:
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Rong Guo; Dongyi Xu; Weidong Wang |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Review Date: 2009-02-24 |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: 48 ISSN: 1095-9130 ISO Abbreviation: Methods Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-05-27 Completed Date: 2009-08-13 Revised Date: 2010-09-23 |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: United States |
Other Details:
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Languages: eng Pagination: 72-9 Citation Subset: IM |
Affiliation:
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Laboratory of Genetics, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, Baltimore, MD 21224, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bloom Syndrome
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genetics*,
metabolism Carrier Proteins / analysis*, isolation & purification, metabolism DNA Damage* DNA Topoisomerases, Type I / analysis*, isolation & purification, metabolism DNA-Binding Proteins / analysis*, isolation & purification, metabolism Fanconi Anemia / genetics*, metabolism Humans Nuclear Proteins / analysis*, isolation & purification, metabolism RecQ Helicases / analysis*, isolation & purification, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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Z01 AG000657-08/AG/NIA NIH HHS; Z01 AG000657-08/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/DNA-Binding Proteins; 0/Nuclear Proteins; 0/RMI1 protein, human; 0/RMI2 protein, human; EC 3.6.1.-/Bloom syndrome protein; EC 3.6.1.-/RecQ Helicases; EC 5.99.1.2/DNA Topoisomerases, Type I; EC 5.99.1.2/topoisomerase IIIalpha |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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