| Identification of amylase isoenzymes in intestinal contents. | |
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MedLine Citation:
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PMID: 6200278 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Samples of intestinal contents were obtained from 16 patients: 5 from the midjejunum, 10 from the distal ileum, and 1 from the cecum. The presence of pancreatic and nonpancreatic isoamylases in these fluids was evaluated by an inhibitor assay (Pharmacia Corporation) and by polyacrylamide gel electrophoresis. In every sample the inhibitor assay detected the presence of pancreatic-type isoamylase but not salivary-type isoamylase. Electrophoresis confirmed that salivary-type isoamylase was totally absent. Therefore, in intestinal disorders which disrupt the intestinal mucosal barrier (such as ischemia, perforation, ulceration, or obstruction), the amylase which escapes from the intestinal lumen and is reabsorbed into the circulation is likely to consist entirely of pancreatic-type isoamylases. In these circumstances, measurement of serum amylase isoenzymes will not distinguish acute pancreatitis from an acute intraabdominal catastrophe requiring surgery. |
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Authors:
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P A Banks; A L Warshaw; G Z Wolfe; A Engalichev; D Duchainey |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Digestive diseases and sciences Volume: 29 ISSN: 0163-2116 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 1984 Apr |
Date Detail:
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Created Date: 1984-05-11 Completed Date: 1984-05-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 297-9 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amylases
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analysis* Cecum Diagnosis, Differential Female Humans Ileum Intestinal Diseases / diagnosis Isoenzymes / analysis* Jejunum Male Pancreatitis / diagnosis Salivary Glands / enzymology |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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