Document Detail

Identification of the actin and plasminogen binding regions of group B streptococcal phosphoglycerate kinase.
MedLine Citation:
PMID:  22761440     Owner:  NLM     Status:  MEDLINE    
Phosphoglycerate kinase (PGK), present on the surface of group B streptococcus (GBS), has previously been demonstrated to bind the host proteins actin and plasminogen. The actin and plasminogen binding sites of GBS-PGK were identified using truncated GBS-PGK molecules, followed by peptide mapping. These experiments identified two actin and plasminogen binding sites located between amino acids 126-134 and 204-208 of the 398-amino acid-long GBS-PGK molecule. Substitution of the lysine residues within these regions with alanine resulted in significantly reduced binding to both actin and plasminogen. In addition, conversion of the glutamic acid residue at amino acid 133 to proline, the amino acid found at this position for the PGK protein of Streptococcus pneumoniae, also resulted in significantly reduced binding to actin and plasminogen. These results demonstrate that the lysine residues at amino acid positions 126, 127, 130, 204, and 208 along with the glutamic acid residue at amino acid position 133 are necessary for actin and plasminogen binding by GBS-PGK.
Tyler J Boone; Gregory J Tyrrell
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-03
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  287     ISSN:  1083-351X     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-08-20     Completed Date:  2012-10-31     Revised Date:  2013-08-20    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  29035-44     Citation Subset:  IM    
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
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MeSH Terms
Actins / chemistry*,  genetics,  metabolism
Bacterial Proteins / chemistry*,  genetics,  metabolism
Peptide Mapping / methods
Phosphoglycerate Kinase / chemistry*,  genetics,  metabolism
Plasminogen / chemistry*,  genetics,  metabolism
Protein Binding
Streptococcus agalactiae / enzymology*,  genetics
Streptococcus pneumoniae / enzymology,  genetics
Reg. No./Substance:
0/Actins; 0/Bacterial Proteins; 9001-91-6/Plasminogen; EC Kinase

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