| Identification of the actin and plasminogen binding regions of group B streptococcal phosphoglycerate kinase. | |
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MedLine Citation:
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PMID: 22761440 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phosphoglycerate kinase (PGK), present on the surface of group B streptococcus (GBS), has previously been demonstrated to bind the host proteins actin and plasminogen. The actin and plasminogen binding sites of GBS-PGK were identified using truncated GBS-PGK molecules, followed by peptide mapping. These experiments identified two actin and plasminogen binding sites located between amino acids 126-134 and 204-208 of the 398-amino acid-long GBS-PGK molecule. Substitution of the lysine residues within these regions with alanine resulted in significantly reduced binding to both actin and plasminogen. In addition, conversion of the glutamic acid residue at amino acid 133 to proline, the amino acid found at this position for the PGK protein of Streptococcus pneumoniae, also resulted in significantly reduced binding to actin and plasminogen. These results demonstrate that the lysine residues at amino acid positions 126, 127, 130, 204, and 208 along with the glutamic acid residue at amino acid position 133 are necessary for actin and plasminogen binding by GBS-PGK. |
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Authors:
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Tyler J Boone; Gregory J Tyrrell |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-07-03 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 287 ISSN: 1083-351X ISO Abbreviation: J. Biol. Chem. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-08-20 Completed Date: 2012-10-31 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 29035-44 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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chemistry*,
genetics,
metabolism Bacterial Proteins / chemistry*, genetics, metabolism Humans Peptide Mapping / methods Phosphoglycerate Kinase / chemistry*, genetics, metabolism Plasminogen / chemistry*, genetics, metabolism Protein Binding Streptococcus agalactiae / enzymology*, genetics Streptococcus pneumoniae / enzymology, genetics |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Bacterial Proteins; 9001-91-6/Plasminogen; EC 2.7.2.3/Phosphoglycerate Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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