Document Detail


Identification of X-linked genes required for migration and programmed cell death of Drosophila melanogaster germ cells.
MedLine Citation:
PMID:  12242239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Drosophila germ cells form at the posterior pole of the embryo and migrate to the somatic gonad. Approximately 50% of the germ cells that form reach their target. The errant cells within the embryo undergo developmentally regulated cell death. Prior studies have identified some autosomal genes that regulate germ cell migration, but the genes that control germ cell death are not known. To identify X-linked genes required for germ cell migration and/or death, we performed a screen for mutations that disrupt these processes. Here we report the identification of scattershot and outsiders, two genes that regulate the programmed death of germ cells. The scattershot gene is defined by a mutation that disrupts both germ cell migration and the death of germ cells ectopic to the gonad. Maternal and zygotic expression of scattershot is required, but the migration and cell death functions can be genetically uncoupled. Zygotic expression of wild-type scattershot rescues germ cell pathfinding, but does not restore the programmed death of errant cells. The outsiders gene is required zygotically. In outsiders mutant embryos, the appropriate number of germ cells is incorporated into the gonad, but germ cells ectopic to the gonad persist.
Authors:
Clark R Coffman; Rachel C Strohm; Fredrick D Oakley; Yukiko Yamada; Danielle Przychodzin; Robert E Boswell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genetics     Volume:  162     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-20     Completed Date:  2003-03-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-84     Citation Subset:  IM    
Affiliation:
Department of Zoology and Genetics, Iowa State University, Ames, Iowa 50011-3260, USA. ccoffman@iastate.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / genetics*
Cell Movement / genetics*
Drosophila melanogaster / cytology,  genetics*
Genetic Complementation Test
Germ Cells / cytology*
Linkage (Genetics)*
X Chromosome*
Grant Support
ID/Acronym/Agency:
GM-57989/GM/NIGMS NIH HHS
Comments/Corrections

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