Document Detail

Identification of a Theileria annulata antigen expressed in multiple stages of the parasite life cycle.
MedLine Citation:
PMID:  9709037     Owner:  NLM     Status:  MEDLINE    
In order to identify sporozoite surface molecules which may be involved in invasion and could act as potential vaccine candidates, a number of Mabs were raised in mice against T. annulata sporozoites. These were assayed for their ability to block sporozoite invasion of bovine peripheral blood mononuclear (PBM) cells in vitro. One of these, Mab 4B11, was found to neutralize sporozoite invasion to a high degree and to recognize a group of sporozoite antigens on Western blots. A T. annulata lambdagt11 genomic expression library was screened with Mab 4B11 and a positive clone containing a 900-bp insert (KP8) analysed further. Data from Southern and Northern blotting indicated that the gene containing the KP8 sequence, termed sporozoite and macroschizont gene 2 (spm2), was expressed both in T. annulata sporozoites and in later parasite life-cycle stages, macroschizont-infected leucocytes and piroplasms. The KP8 sequence was expressed in E. coli as a fusion protein with glutathione-S-transferase (GST) using the vector pGEX1lambdaT. Bovine antiserum raised against GST-KP8 recognised a single high molecular weight molecule on Western blots corresponding to one of the antigens recognised by Mab 4B11, expressed in sporozoites, macroschizont-infected leucocytes, and piroplasms. While our evidence suggests that the spm2 molecule alone is not responsible for sporozoite neutralization, it is a multistage antigen likely to function both in T. annulata sporozoites and in subsequent parasite life-cycle stages.
P A Knight; S W Williamson; C G Brown; L Bell-Sakyi; E Kirvar; J Kinnaird; K Hussain; R Hall; N Boulter; A Tait
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental parasitology     Volume:  90     ISSN:  0014-4894     ISO Abbreviation:  Exp. Parasitol.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-09-11     Completed Date:  1998-09-11     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0370713     Medline TA:  Exp Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  110-21     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Centre for Tropical Veterinary Medicine, Veterinary Field Station, University of Edinburgh, Easter Bush, Roslin, Midlothian, Edinburgh, EH25 9RG, UK.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal
Antibody Specificity
Antigens, Protozoan / biosynthesis*,  chemistry,  immunology
Base Sequence
Blotting, Western
Genomic Library
Glutathione Transferase / biosynthesis
Leukocytes, Mononuclear / immunology,  parasitology
Life Cycle Stages
Molecular Sequence Data
Neutralization Tests
Recombinant Fusion Proteins / biosynthesis,  chemistry,  immunology
Theileria annulata / growth & development*,  immunology,  pathogenicity
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Protozoan; 0/KP8 antigen; 0/Recombinant Fusion Proteins; EC Transferase

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