| Identification of TPO receptors on central nervous system-a preliminary report. | |
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MedLine Citation:
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PMID: 15363139 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR, MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells. |
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Authors:
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Mo Yang; Wen-Jie Xia; Karen Li; Nga-Hin Pong; Ki-Wai Chik; Chi-Kong Li; Margaret H L Ng; Ho-Keung Ng; Kwok-Pui Fung; Tai-Fai Fok |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology Volume: 12 ISSN: 1009-2137 ISO Abbreviation: Zhongguo Shi Yan Xue Ye Xue Za Zhi Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-09-14 Completed Date: 2005-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 101084424 Medline TA: Zhongguo Shi Yan Xue Ye Xue Za Zhi Country: China |
Other Details:
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Languages: eng Pagination: 494-7 Citation Subset: IM |
Affiliation:
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Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong. yang1091@cuhk.edu.hk |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects Brain Chemistry* Cell Line Cell Proliferation / drug effects Erythropoietin / pharmacology Humans Mice Neoplasm Proteins / analysis* Neurons / drug effects Oncogene Proteins / analysis* Proto-Oncogene Proteins / analysis* Receptors, Cytokine / analysis* Receptors, Thrombopoietin Thrombopoietin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Mpl protein, mouse; 0/Neoplasm Proteins; 0/Oncogene Proteins; 0/Proto-Oncogene Proteins; 0/Receptors, Cytokine; 0/Receptors, Thrombopoietin; 11096-26-7/Erythropoietin; 143641-95-6/MPL protein, human; 9014-42-0/Thrombopoietin |
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