Document Detail

Identification of TPO receptors on central nervous system-a preliminary report.
MedLine Citation:
PMID:  15363139     Owner:  NLM     Status:  MEDLINE    
To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR, MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.
Mo Yang; Wen-Jie Xia; Karen Li; Nga-Hin Pong; Ki-Wai Chik; Chi-Kong Li; Margaret H L Ng; Ho-Keung Ng; Kwok-Pui Fung; Tai-Fai Fok
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology     Volume:  12     ISSN:  1009-2137     ISO Abbreviation:  Zhongguo Shi Yan Xue Ye Xue Za Zhi     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-09-14     Completed Date:  2005-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101084424     Medline TA:  Zhongguo Shi Yan Xue Ye Xue Za Zhi     Country:  China    
Other Details:
Languages:  eng     Pagination:  494-7     Citation Subset:  IM    
Department of Paediatrics, The Chinese University of Hong Kong, Hong Kong.
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MeSH Terms
Apoptosis / drug effects
Brain Chemistry*
Cell Line
Cell Proliferation / drug effects
Erythropoietin / pharmacology
Neoplasm Proteins / analysis*
Neurons / drug effects
Oncogene Proteins / analysis*
Proto-Oncogene Proteins / analysis*
Receptors, Cytokine / analysis*
Receptors, Thrombopoietin
Thrombopoietin / pharmacology
Reg. No./Substance:
0/Mpl protein, mouse; 0/Neoplasm Proteins; 0/Oncogene Proteins; 0/Proto-Oncogene Proteins; 0/Receptors, Cytokine; 0/Receptors, Thrombopoietin; 11096-26-7/Erythropoietin; 143641-95-6/MPL protein, human; 9014-42-0/Thrombopoietin

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