| Identification of T lymphocytes, macrophages, and activated eosinophils in the bronchial mucosa in intrinsic asthma. Relationship to symptoms and bronchial responsiveness. | |
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MedLine Citation:
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PMID: 1489147 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using immunohistochemistry and a panel of monoclonal antibodies, we have compared T-lymphocyte, eosinophil, macrophage, and neutrophil infiltration in bronchial biopsies from 10 intrinsic (nonallergic) asthmatics (IA) and seven extrinsic (allergic) asthmatic (EA), with similar degrees of disease severity. The results were compared with 12 normal healthy nonatopic controls (NC). All subjects were nonsmokers and were not taking oral or inhaled corticosteroids. An intense mononuclear cell infiltrate was identified in IA with an increase in the number of CD45+ cells (total leukocytes), CD3+ and CD4+ lymphocytes, and CD68+ macrophages (p < 0.03, p < 0.01, p < 0.03, and p < 0.03, respectively), compared with NC. Increases were also found in CD4+ (p < 0.05) and CD68+ (p < 0.05) cell numbers between IA and EA. IL-2 receptor-bearing cells (CD25+) and the number of total (MBP+) and actively secreting (EG2+) eosinophils, were also increased in IA compared with NC (p < 0.01, p < 0.01, and p < 0.01, respectively). Similar increases in EG2+ eosinophils and CD25+ (IL-2 receptor-positive) cells were observed in EA (p < 0.01 and p < 0.02, respectively). No differences were detected in the three groups for the number of elastase-positive cells (neutrophils). EG2+ numbers in IA correlated with the Aas asthma symptoms score (r = 0.65, p < 0.05), whereas EG2+ cell numbers in all asthmatics (IA + EA) correlated with airway methacholine responsiveness (r = -0.55, p < 0.03) and with the Aas asthma symptom score (r = 0.54, p < 0.03).(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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A M Bentley; G Menz; C Storz; D S Robinson; B Bradley; P K Jeffery; S R Durham; A B Kay |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The American review of respiratory disease Volume: 146 ISSN: 0003-0805 ISO Abbreviation: Am. Rev. Respir. Dis. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1993-02-24 Completed Date: 1993-02-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370523 Medline TA: Am Rev Respir Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 500-6 Citation Subset: AIM; IM |
Affiliation:
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Department of Allergy and Clinical Immunology, National Heart & Lung Institute, London, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Asthma / complications, immunology, pathology* Biopsy Bronchi / chemistry*, immunology, pathology Bronchial Hyperreactivity / complications, immunology, pathology* Bronchial Provocation Tests Eosinophils / chemistry* Evaluation Studies as Topic Female Forced Expiratory Volume Humans Hypersensitivity, Immediate / complications Immunohistochemistry Macrophages / chemistry* Male Methacholine Chloride / diagnostic use Middle Aged Mucous Membrane / chemistry, immunology, pathology Neutrophils / chemistry* Severity of Illness Index T-Lymphocyte Subsets T-Lymphocytes / chemistry* |
| Chemical | |
Reg. No./Substance:
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62-51-1/Methacholine Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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