| Identification of Streptococcus intermedius Central Nervous System Infection using PCR and Electrospray Ionization Mass Spectrometry. | |
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MedLine Citation:
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PMID: 23035188 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We describe the utility of PCR and electrospray-ionization with mass spectrometry (PCR/ESI-MS) of "culture negative" cerebrospinal fluid (CSF) in order to identify Gram-positive cocci noted on a Gram stain of CSF from a previously healthy 26-year-old man with community acquired pneumonia (CAP) and multiple brain abscesses. CSF samples were obtained two weeks apart, first by lumbar puncture and two weeks later from an external ventricular drain that was inserted into the right ventricle. Both CSF cultures were negative. Gram stain of Bronchioalveolar lavage (BAL) fluid was notable for many Gram positive cocci (GPC), but cultures of BAL fluid and subcarinal lymph node biopsy tissue were negative. PCR/ESI-MS detected Streptococcus intermedius, a common cause of brain abscesses, in both CSF samples as well as in the fixed tissue from the biopsy. This unique case confirms S. intermedius pulmonary infection as the source of metastatic CNS infection and reveals the potential of PCR/ESI-MS to detect a streptococcal pathogen not captured by conventional cultures. |
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Authors:
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Nisha S Bhatia; John J Farrell; Rangarajan Sampath; Raymond Ranken; Megan A Rounds; David J Ecker; Robert A Bonomo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: - ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Illinois Neurological Institute, Peoria, IL. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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