Document Detail


Identification of Shiga-like toxin Escherichia coli isolated from children with diarrhea by polymerase chain reaction.
MedLine Citation:
PMID:  12123543     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To evaluate the etiology of Shiga like toxin producing Escherichia coli (SLTEC) in children with diarrhea. METHODS: We designed and synthesized 3 pairs of primers located in the SLT1, SLT2, and eaeA genes of enterohaemorrhagic Escherichia coli (EHEC), while the virulent genes SLT1, SLT2, and eaeA from E.coli species were amplified by polymerase chain reaction (PCR). RESULTS: One strain of EHEC with SLT1, SLT2, and eaeA in 29 reference strains of diarrhea-causing E.coli (DCEC) and 10 strains of other enterobacteria detected by PCR had positive reactions, while all other DCEC and enterobacteria were negative. Of 474 strains of E. coli isolated from 1032 children with diarrhea and detected by PCR, 20 strains of SLT1 producing E. coli (4.2%) positive, and 7 strains of SLT2 producing E. coli (1.5%) positive; while of 74 strains of entero-SLTs-producing and invasive Escherichia coli (ESIEC), 15 strains of SLT1 (20.3%) and 5 strains of SLT2 (6.8%) were positive. CONCLUSION: Shiga-like toxin E. coli has been identified as a major etiologic agent of children with diarrhea in Taiyuan, China.
Authors:
Qingyi Zhu; Lianqing Li; Zhaobiao Guo; Ruifu Yang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chinese medical journal     Volume:  115     ISSN:  0366-6999     ISO Abbreviation:  Chin. Med. J.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-07-18     Completed Date:  2002-08-13     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7513795     Medline TA:  Chin Med J (Engl)     Country:  China    
Other Details:
Languages:  eng     Pagination:  815-8     Citation Subset:  IM    
Affiliation:
Laboratory of Molecular Microbiology, Shanxi Children's Hospital, Taiyuan 030013, China.
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MeSH Terms
Descriptor/Qualifier:
Child
Diarrhea / microbiology*
Escherichia coli / classification,  isolation & purification*,  pathogenicity
Feces / microbiology
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Shiga Toxins / genetics*
Chemical
Reg. No./Substance:
0/Shiga Toxins

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