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Identification of Serum Protein Biomarkers in Biliary Atresia by Mass Spectrometry and ELISA.
MedLine Citation:
PMID:  22569524     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
OBJECTIVE:: There is an urgent need to identify noninvasive and convenient biomarkers for early diagnosis of biliary atresia (BA). The aim of this study was to identify potential protein biomarkers for BA. PATIENTS AND METHODS:: Serum samples from 42 BA infants, 38 non-BA neonatal cholestasis infants (NC), and 36 healthy controls (HC) were randomly divided into a training set and a test set. Serum proteomic profiles were measured using SELDI-TOF-MS. Candidate biomarkers were purified using HPLC, identified using LC-MS/MS, and validated using ELISA. RESULTS:: A total of 2 protein peaks (m/z with 8697 and 9098 Da) with differential expression levels were found using SELDI-TOF-MS. These peaks were then analyzed by a support vector machine to construct a classification model in the training set. The sensitivity and specificity of the model were 94.1% and 91.8%, respectively, in the test set. One candidate biomarker (9098 Da) was identified as Apo C-II, and was found to be down-regulated in BA samples compared with HC samples, and up-regulated in BA samples compared to NC samples. The other candidate biomarker (8697 Da) was identified as Apo C-III, and was found to be up-regulated in BA compared with NC and HC. CONCLUSIONS:: Apo C-II and Apo C-III may be potential protein biomarkers of BA and may be usefull in distinguishing BA infants from healthy and NC infants. Further studies with additional populations or using prediagnostic serum are needed to confirm the importance of these findings as diagnostic markers of BA infants.
Authors:
Zai Song; Rui Dong; Yuxia Fan; Shan Zheng
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-7
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  -     ISSN:  1536-4801     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
*Department of Pediatric Surgery, Children's Hospital of Fudan University, and Key Laboratory of Neonatal Disease, Ministry of Health, 399 Wan Yuan Road, Shanghai 201102, China †Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, 1 Jian She Road, Zhengzhou 450052, China.
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