Document Detail


Identification of Salmonellae with the 4-methylumbelliferyl caprilate fluorescence test.
MedLine Citation:
PMID:  1774276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have tested 750 Salmonella strains and 130 strains of other species of the family Enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (MUCAP) test. The MUCAP test is a fluorescence test for rapid identification of Salmonella strains. The non-Salmonella strains were strains sent for identification as suspected Salmonella strains and thus have phenotypes similar to those of Salmonella strains. All 748 tested Salmonella strains of subgroups I, II, III, and IV were positive in the MUCAP test. Of the two tested rare Salmonella subgroup V strains, one was positive and the other was negative. In the selected material containing strains with phenotypes similar to those of Salmonella strains, only one Hafnia alvei strain of 130 Enterbacteriaceae bacteria tested was positive. The fluorescence of the H. alvei strain, the six tested Salmonella dublin strains, and the positive Salmonella subgroup V strain was weaker than that of the other salmonellae. The MUCAP assay is simple and is performed within 5 min. With an almost 100% sensitivity for Salmonella strains, apart from a single Salmonella subgroup V strain, we found the MUCAP test to be a convenient complement to traditional biochemical identification methods, especially for atypical and unusual Salmonella strains.
Authors:
M Olsson; A Syk; R Wollin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  29     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-03-05     Completed Date:  1992-03-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2631-2     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, National Bacteriological Laboratory, Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques*
Enterobacteriaceae / classification,  isolation & purification
Evaluation Studies as Topic
Fluorescent Dyes
Humans
Hymecromone / analogs & derivatives*
Salmonella / classification,  isolation & purification*
Serotyping
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 20671-66-3/4-methylumbelliferyl caprylate; 90-33-5/Hymecromone
Comments/Corrections

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