Document Detail

Identification of a Saccharomyces cerevisiae gene that is required for G1 arrest in response to the lipid oxidation product linoleic acid hydroperoxide.
MedLine Citation:
PMID:  11408586     Owner:  NLM     Status:  MEDLINE    
Reactive oxygen species cause damage to all of the major cellular constituents, including peroxidation of lipids. Previous studies have revealed that oxidative stress, including exposure to oxidation products, affects the progression of cells through the cell division cycle. This study examined the effect of linoleic acid hydroperoxide, a lipid peroxidation product, on the yeast cell cycle. Treatment with this peroxide led to accumulation of unbudded cells in asynchronous populations, together with a budding and replication delay in synchronous ones. This observed modulation of G1 progression could be distinguished from the lethal effects of the treatment and may have been due to a checkpoint mechanism, analogous to that known to be involved in effecting cell cycle arrest in response to DNA damage. By examining several mutants sensitive to linoleic acid hydroperoxide, the YNL099c open reading frame was found to be required for the arrest. This gene (designated OCA1) encodes a putative protein tyrosine phosphatase of previously unknown function. Cells lacking OCA1 did not accumulate in G1 on treatment with linoleic acid hydroperoxide, nor did they show a budding, replication, or Start delay in synchronous cultures. Although not essential for adaptation or immediate cellular survival, OCA1 was required for growth in the presence of linoleic acid hydroperoxide, thus indicating that it may function in linking growth, stress responses, and the cell cycle. Identification of OCA1 establishes cell cycle arrest as an actively regulated response to oxidative stress and will enable further elucidation of oxidative stress-responsive signaling pathways in yeast.
N Alic; V J Higgins; I W Dawes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biology of the cell     Volume:  12     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-06-15     Completed Date:  2001-08-23     Revised Date:  2013-04-17    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1801-10     Citation Subset:  IM    
School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, New South Wales 2052, Australia.
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MeSH Terms
Amino Acid Sequence
Cell Cycle
Cells, Cultured
DNA / metabolism
DNA Damage
Dose-Response Relationship, Drug
G1 Phase*
Hydrogen Peroxide / metabolism
Linoleic Acids / metabolism*
Lipid Metabolism*
Lipid Peroxides / metabolism*
Molecular Sequence Data
Open Reading Frames
Oxygen / metabolism*
Saccharomyces cerevisiae / genetics*
Sequence Homology, Amino Acid
Signal Transduction
Time Factors
Reg. No./Substance:
0/Linoleic Acids; 0/Lipid Peroxides; 25657-09-4/linoleic acid hydroperoxide; 7722-84-1/Hydrogen Peroxide; 7782-44-7/Oxygen; 9007-49-2/DNA

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