| Identification and Ruminal Outflow of Long-Chain Fatty Acid Biohydrogenation Intermediates in Cows Fed Diets Containing Fish Oil. | |
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MedLine Citation:
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PMID: 21562931 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The abundance of 20- to 24-carbon fatty acids in omasal digesta of cows fed grass silage-based diets supplemented with 0 (Control) and 250 g/day of fish oil (FO) was examined to investigate the fate of long-chain unsaturated fatty acids in the rumen. Complimentary argentation thin-layer chromatography and gas-chromatography mass-spectrometry analysis of fatty acid methyl esters and corresponding 4,4-dimethyloxazoline derivatives prepared from fish oil and omasal digesta enabled the structure of novel 20- to 22-carbon fatty acids to be elucidated. Compared with the Control, the FO treatment resulted in the formation and accumulation of 27 novel 20- and 22-carbon biohydrogenation intermediates containing at least one trans double bond and the appearance of cis-14 20:1, 20:2n-3, 21:4n-3 and 22:3n-6 not contained in fish oil. No conjugated ≥20-carbon fatty acids were detected in Control or FO digesta. In conclusion, fish oil in the diet results in the formation of numerous long-chain biohydrogenation intermediates in the rumen of lactating cows. Comparison of the intake and flow of 20-, 21- and 22-carbon fatty acids at the omasum in cows fed the Control and FO treatments suggests that the first committed steps of 20:5n-3, 21:5n-3 and 22:6n-3 hydrogenation in the rumen involve the reduction and/or isomerisation of double bonds closest to the carboxyl group. |
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Authors:
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Piia Kairenius; Vesa Toivonen; Kevin J Shingfield |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-12 |
Journal Detail:
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Title: Lipids Volume: - ISSN: 1558-9307 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0060450 Medline TA: Lipids Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Animal Production Research, MTT Agrifood Research Finland, 31600, Jokioinen, Finland. |
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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