| Identification of ROS using oxidized DCFDA and flow-cytometry. | |
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MedLine Citation:
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PMID: 20072909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive production of ROS. Oxidative stress occurs in cells when the generation of ROS overwhelms the cells' natural antioxidant defenses. ROS and the oxidative damage are thought to play an important role in many human diseases including cancer, atherosclerosis, other neurodegenerative diseases and diabetes. Thus, establishing their precise role requires the ability to measure ROS accurately and the oxidative damage that they cause. There are many methods for measuring free radical production in cells. The most straightforward techniques use cell permeable fluorescent and chemiluminescent probes. 2'-7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is one of the most widely used techniques for directly measuring the redox state of a cell. It has several advantages over other techniques developed. It is very easy to use, extremely sensitive to changes in the redox state of a cell, inexpensive and can be used to follow changes in ROS over time. |
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Authors:
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Evgeniy Eruslanov; Sergei Kusmartsev |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 594 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 57-72 Citation Subset: IM |
Affiliation:
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Shands Cancer Center and Department of Urology, University of Florida, Gainesville, FL, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Flow Cytometry / methods* Fluoresceins / chemistry* Humans Mice Mice, Nude Oxidative Stress / physiology* Reactive Oxygen Species / chemistry*, metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Reactive Oxygen Species; 2044-85-1/diacetyldichlorofluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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