Document Detail


Identification of RGS1 as a candidate biomarker for undifferentiated spondylarthritis by genome-wide expression profiling and real-time polymerase chain reaction.
MedLine Citation:
PMID:  19877080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To compare gene expression profiles between ankylosing spondylitis (AS) and undifferentiated spondylarthritis (uSpA) patients with inflammatory low back pain.
METHODS: Peripheral blood mononuclear cells (PBMCs) from patients with AS, patients with uSpA, and healthy subjects were screened using genome-wide microarrays, followed by validation by real-time polymerase chain reaction (PCR).
RESULTS: Microarray profiling and real-time PCR assays showed only minor differences between AS patients and healthy subjects. In contrast, 20 genes were strikingly more highly expressed in uSpA patients. Regulator of G protein signaling 1 (RGS1) was identified as the most useful biomarker for distinguishing uSpA patients, and to a lesser extent AS patients, from control subjects (P = 2.3 x 10(-7) and 6.7 x 10(-3), respectively). These findings were verified in an independent cohort that also included patients with rheumatoid arthritis and patients with mechanical low back pain. The receiver operating characteristic area under the curve values in the first and second cohorts of uSpA patients were 0.99 and 0.93, respectively (P = 1 x 10(-4)). To evaluate the possible derivation of RGS1, we cultured a monocyte-derived cell line with a panel of cytokines and chemokines. RGS1 was significantly induced either by tumor necrosis factor alpha (TNFalpha) or by interleukin-17 (IL-17).
CONCLUSION: Our findings indicate that uSpA PBMCs carry strikingly more highly expressed genes compared with PBMCs from AS patients or healthy subjects, and that TNFalpha- and IL-17-inducible RGS1 is a potential biomarker for uSpA, and to a lesser extent for AS, with inflammatory low back pain.
Authors:
Jieruo Gu; Yu-Ling Wei; James C C Wei; Feng Huang; Ming-Shiou Jan; Michael Centola; Mark B Frank; David Yu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  60     ISSN:  0004-3591     ISO Abbreviation:  Arthritis Rheum.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-10     Completed Date:  2010-01-04     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3269-79     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / blood
Case-Control Studies
Cells, Cultured
Diagnosis, Differential
Female
Gene Expression Profiling*
Humans
Interleukin-17 / pharmacology
Leukocytes, Mononuclear / drug effects,  metabolism,  pathology
Male
Middle Aged
RGS Proteins / blood*
Reverse Transcriptase Polymerase Chain Reaction*
Spondylarthritis / blood*,  diagnosis*
Spondylitis, Ankylosing / blood,  diagnosis
Tumor Necrosis Factor-alpha / pharmacology
Grant Support
ID/Acronym/Agency:
5U-19-AI062629/AI/NIAID NIH HHS; AR061-015/AR/NIAMS NIH HHS; AR081-006/AR/NIAMS NIH HHS; MC-P20-RR-15577/RR/NCRR NIH HHS; P20 RR015577/RR/NCRR NIH HHS; P20 RR015577-086790/RR/NCRR NIH HHS; P20 RR015577-096179/RR/NCRR NIH HHS; P20 RR016478/RR/NCRR NIH HHS; P20 RR016478-077281/RR/NCRR NIH HHS; P20 RR016478-087197/RR/NCRR NIH HHS; P20 RR016478-096180/RR/NCRR NIH HHS; P20 RR020143-047652/RR/NCRR NIH HHS; P20-RR-016478/RR/NCRR NIH HHS; P20-RR-020143/RR/NCRR NIH HHS; P20-RR-15577/RR/NCRR NIH HHS; P30 AR053483/AR/NIAMS NIH HHS; U19 AI062629/AI/NIAID NIH HHS; U19 AI062629-040006/AI/NIAID NIH HHS; U19-AI-062629/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Interleukin-17; 0/RGS Proteins; 0/RGS1 protein, human; 0/Tumor Necrosis Factor-alpha
Comments/Corrections

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