Document Detail


Identification of Pseudomonas aeruginosa genes involved in virulence and anaerobic growth.
MedLine Citation:
PMID:  16790798     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pseudomonas aeruginosa is a gram-negative, opportunistic pathogen and a significant cause of acute and chronic infections in patients with compromised host defenses. Evidence suggests that within infections P. aeruginosa encounters oxygen limitation and exists in microbial aggregates known as biofilms. However, there is little information that describes genes involved in anaerobic growth of P. aeruginosa and their association with virulence of this pathogen. To identify genes required for anaerobic growth, random transposon (Tn) mutagenesis was used to screen for mutants that demonstrated the inability to grow anaerobically using nitrate as a terminal electron acceptor. Of approximately 35,000 mutants screened, 57 mutants were found to exhibit no growth anaerobically using nitrate. Identification of the genes disrupted by the Tn revealed 24 distinct loci required for anaerobic growth on nitrate, including several genes not previously associated with anaerobic growth of P. aeruginosa. Several of these mutants were capable of growing anaerobically using nitrite and/or arginine, while five mutants were unable to grow anaerobically under any of the conditions tested. Three mutants were markedly attenuated in virulence in the lettuce model of P. aeruginosa infection. These studies have identified novel genes important for anaerobic growth and demonstrate that anaerobic metabolism influences virulence of P. aeruginosa.
Authors:
Melanie J Filiatrault; Kristin F Picardo; Helen Ngai; Luciano Passador; Barbara H Iglewski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  74     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-08-24     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4237-45     Citation Subset:  IM    
Affiliation:
University of Rochester School of Medicine and Dentistry, Department of Microbiology and Immunology, 601 Elmwood Avenue, Box 672, Rochester, NY 14642, USA.
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MeSH Terms
Descriptor/Qualifier:
Anaerobiosis
Lettuce / microbiology
Oxygen / metabolism*
Pseudomonas Infections / microbiology
Pseudomonas aeruginosa / genetics*,  growth & development,  pathogenicity*
Virulence
Grant Support
ID/Acronym/Agency:
F32AI056825/AI/NIAID NIH HHS; R37AI33713/AI/NIAID NIH HHS; T32AI07362/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
7782-44-7/Oxygen
Comments/Corrections

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