| Identification, proliferation, and differentiation of adult Leydig stem cells. | |
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MedLine Citation:
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PMID: 22865373 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leydig cells, the testosterone-producing cells of the adult testis, rarely turn over. However, their elimination with ethane dimethanesulfonate (EDS) is followed by the appearance of new, fully functional adult Leydig cells. The cells that give rise to the new Leydig cells have not been well characterized, and little is known about the mechanism by which they are regulated. We isolated cells expressing platelet-derived growth factor receptor-α, but not 3β-hydroxysteroid dehydrogenase (3β-HSD(neg)) from the testes of EDS-treated adult rats. Depending on conditions, these cells proliferated indefinitely or differentiated and produced testosterone. To localize these cells and to determine the effect of the testicular environment on their function, the seminiferous tubules and testicular interstitium were physically separated and cultured. During the first 72 h in culture, 3β-HSD(neg) cells on the tubule surfaces underwent divisions. Some of these cells later expressed 3β-HSD and produced testosterone. Removal of the newly formed 3β-HSD(pos) cells from the tubule surfaces with EDS, followed by further culture of the stripped tubules, resulted in the reappearance of testosterone-producing cells. These results, taken together, suggest that the precursors for newly formed Leydig cells are stem cells, with many if not all situated on the surfaces of the seminiferous tubules. Although normally quiescent, the stem cells are capable of self-renewal and differentiation. The development of the tubule culture system should provide a valuable in vitro approach to assess the role(s) of niche components on the function of adult Leydig stem cells despite their residing in a complex mammalian tissue. |
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Authors:
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Erin Stanley; Chieh-Yin Lin; Shiying Jin; June Liu; Chantal M Sottas; Renshan Ge; Barry R Zirkin; Haolin Chen |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-08-03 |
Journal Detail:
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Title: Endocrinology Volume: 153 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-09-24 Completed Date: 2012-12-11 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 5002-10 Citation Subset: AIM; IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland 21205, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Stem Cells
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cytology*,
metabolism Animals Cell Differentiation / drug effects, physiology* Cell Proliferation / drug effects* Cells, Cultured Leydig Cells / cytology*, drug effects, metabolism Luteinizing Hormone / pharmacology Male Rats Rats, Inbred BN Testosterone / biosynthesis |
| Grant Support | |
ID/Acronym/Agency:
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R01 AG030598/AG/NIA NIH HHS; R01 HD050570/HD/NICHD NIH HHS; R03 AG026721/AG/NIA NIH HHS; R37 AG21092/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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58-22-0/Testosterone; 9002-67-9/Luteinizing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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