Document Detail


Identification of Primula "front-end" desaturases with distinct n-6 or n-3 substrate preferences.
MedLine Citation:
PMID:  16773377     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
cDNA clones encoding cytochrome b(5) fusion desaturases were isolated from Primula cortusoides L. and Primula luteola Ruprecht, species previously shown to preferentially accumulate either n-6 or n-3 Delta6-desaturated fatty acids, respectively. Functional characterisation of these desaturases in yeast revealed that the recombinant Primula enzymes displayed substrate preferences, resulting in the predominant synthesis of either gamma-linolenic acid (n-6) or stearidonic acid (n-3). Independent expression of the two Primula desaturases in transgenic Arabidopsis thaliana confirmed these results, with gamma-linolenic acid and stearidonic acid accumulating in both leaf and seed tissues to different levels, depending on the substrate specificity of the desaturase. Targeted lipid analysis of transgenic Arabidopsis lines revealed the presence of Delta6-desaturated fatty acids in the acyl-CoA pools of leaf but not seed tissue. The implications for the transgenic synthesis of C(20) polyunsaturated fatty acids via the elongation of Delta6-desaturated fatty acids are discussed, as is the potential of using Primula desaturases in the synthesis of C(18) n-3 polyunsaturated fatty acids such as stearidonic acid.
Authors:
Olga Sayanova; Richard Haslam; Mónica Venegas-Calerón; Johnathan A Napier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-14
Journal Detail:
Title:  Planta     Volume:  224     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-19     Completed Date:  2007-01-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1269-77     Citation Subset:  IM    
Affiliation:
Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
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MeSH Terms
Descriptor/Qualifier:
Fatty Acid Desaturases / metabolism*
Plants, Genetically Modified
Polymerase Chain Reaction
Primula / enzymology*
Substrate Specificity
Chemical
Reg. No./Substance:
EC 1.14.19.-/Fatty Acid Desaturases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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