Document Detail


Identification of a polo-like kinase 4-dependent pathway for de novo centriole formation.
MedLine Citation:
PMID:  21353560     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Supernumerary centrosomes are a key cause of genomic instability in cancer cells. New centrioles can be generated by duplication with a mother centriole as a platform or, in the absence of preexisting centrioles, by formation de novo. Polo-like kinase 4 (Plk4) regulates both modes of centriole biogenesis, and Plk4 deregulation has been linked to tumor development. We show that Plx4, the Xenopus homolog of mammalian Plk4 and Drosophila Sak, induces de novo centriole formation in vivo in activated oocytes and in egg extracts, but not in immature or in vitro matured oocytes. Both kinase activity and the polo-box domain of Plx4 are required for de novo centriole biogenesis. Polarization microscopy in "cycling" egg extracts demonstrates that de novo centriole formation is independent of Cdk2 activity, a major difference compared to template-driven centrosome duplication that is linked to the nuclear cycle and requires cyclinA/E/Cdk2. Moreover, we show that the Mos-MAPK pathway blocks Plx4-dependent de novo centriole formation before fertilization, thereby ensuring paternal inheritance of the centrosome. The results define a new system for studying the biochemical and molecular basis of de novo centriole formation and centriole biogenesis in general.
Authors:
Frank Eckerdt; Tomomi M Yamamoto; Andrea L Lewellyn; James L Maller
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-02-25
Journal Detail:
Title:  Current biology : CB     Volume:  21     ISSN:  1879-0445     ISO Abbreviation:  Curr. Biol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-07-12     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  428-32     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Cycle Proteins / metabolism*
Centrioles / metabolism*
Microscopy, Polarization
Oocytes / metabolism
Protein-Serine-Threonine Kinases / metabolism*
Signal Transduction / genetics*
Xenopus
Xenopus Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Xenopus Proteins; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 4, Xenopus
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Magnetoreception in an avian brain in part mediated by inner ear lagena.
Next Document:  Longitude Perception and Bicoordinate Magnetic Maps in Sea Turtles.