Document Detail


Identification of Pneumocystis carinii DNA by polymerase chain reaction in necropsy lung samples from children dying of respiratory tract illnesses.
MedLine Citation:
PMID:  11953737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polymerase chain reaction for Pneumocystis carinii DNA was performed on necropsy lung samples from children by means of P carinii -specific primers.P carinii DNA was identified in 22 of 22 (100%) samples with histologically proven P carinii pneumonia and 13 of 75 (17%) with non-P carinii pneumonia respiratory illness (sensitivity, 100%; specificity, 83%). The low specificity precludes the use of polymerase chain reaction as an alternative to histopathologic diagnosis.
Authors:
Francis Kasolo; Kennedy Lishimpi; Chifumbe Chintu; Peter Mwaba; Victor Mudenda; Daniel Maswahu; Hiroshi Terunuma; Helen Fletcher; Andrew Nunn; Sebastian Lucas; Alimuddin Zumla
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pediatrics     Volume:  140     ISSN:  0022-3476     ISO Abbreviation:  J. Pediatr.     Publication Date:  2002 Mar 
Date Detail:
Created Date:  2002-04-15     Completed Date:  2002-04-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375410     Medline TA:  J Pediatr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  367-9     Citation Subset:  AIM; IM    
Affiliation:
UNZA-UCLMS (University of Zambia-University College London Medical School) Tuberculosis/HIV Research and Training Project, the JICA Infectious Diseases Project, Department of Pathology and Microbiology, University Teaching Hospital, Lusaka, Zambia.
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MeSH Terms
Descriptor/Qualifier:
Autopsy
DNA, Bacterial / analysis*
Humans
Lung / microbiology*
Pneumocystis / genetics*,  isolation & purification*
Pneumonia, Pneumocystis / diagnosis*
Polymerase Chain Reaction*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/DNA, Bacterial
Comments/Corrections
Comment In:
J Pediatr. 2002 Mar;140(3):290-2   [PMID:  11953724 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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