| Identification of Oral Bacterial DNA in Synovial Fluid of Patients With Arthritis With Native and Failed Prosthetic Joints. | |
| | |
MedLine Citation:
|
PMID: 22426587 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
OBJECTIVE: We examined the presence of bacterial DNA in synovial fluids of native or aseptically failed prosthetic joints from patients having periodontal disease and arthritis to determine whether there is bacterial spread from the oral cavity to the joints. METHODS: A total of 36 subjects were enrolled in the study. Among these, 11 were diagnosed with rheumatoid arthritis (RA) and 25 were diagnosed with osteoarthritis (OA). Eight patients with OA and 1 patient with RA had failed prostheses. Synovial fluid was aspirated from the affected hip or knee joint. Pooled subgingival plaque samples were collected, followed by clinical periodontal examination. Bacterial DNA was extracted from the collected synovial fluid and dental plaque samples were followed by polymerase chain reactions and DNA sequence analysis of the 16S-23S rRNA genes. RESULTS: Of the 36 patients, bacterial DNA was detected in the synovial fluid samples from 5 patients (13.9%): 2 with RA (1 native and 1 failed prosthetic joints) and 3 with OA (1 native and 2 failed prosthetic joints). Of these 5 patients, 2 were diagnosed with periodontitis and had identical bacterial clones (Fusobacterium nucleatum and Serratia proteamaculans, respectively) detected in both the synovial fluid and the dental plaque samples. Fusobacterium nucleatum was the most prevalent, detected in 4 of the 5 positive samples. CONCLUSIONS: The present findings of bacterial DNA in the synovial fluid suggest the possibility of infection translocating from the periodontal tissue to the synovium. We suggest that patients with arthritis or failed prosthetic joints be examined for the presence of periodontal diseases and be treated accordingly. |
| | |
Authors:
|
Stéphanie Témoin; Alia Chakaki; Ali Askari; Ahmed El-Halaby; Steven Fitzgerald; Randall E Marcus; Yiping W Han; Nabil F Bissada |
Related Documents
:
|
6277807 - Analysis of human genital warts (condylomata acuminata) and other genital tumors for hu... 12565157 - Dna replicative functions of highly-expressed, codon-optimized human papillomavirus pro... 1646197 - Chromosomal insertion and amplification of human papillomavirus 16 dna sequences in a c... 2556927 - Human papillomavirus dna in respiratory papillomatosis detected by in situ hybridizatio... 16689727 - Amplification of low quantity bacterial rna for microarray studies: time-course analysi... 8974107 - The effect of a nucleotide-nucleoside solution on hepatic regeneration after partial he... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-3-15 |
Journal Detail:
|
Title: Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases Volume: - ISSN: 1536-7355 ISO Abbreviation: - Publication Date: 2012 Mar |
Date Detail:
|
Created Date: 2012-3-19 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9518034 Medline TA: J Clin Rheumatol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
From the *Department of Periodontics, School of Dental Medicine, Departments of †Rheumatology and ‡Orthopaedics, School of Medicine, Case Western Reserve University; §Department of Orthopedic Surgery, University Hospitals Case Medical Center; and Departments of ∥Pathology and ¶Reproductive Biology, School of Medicine, Case Western Reserve University, Cleveland, OH. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Are Hospitalized Patients With Fragile Fractures Managed Properly in Relation to Underlying Osteopor...
Next Document: False-Negative Dual-Energy Computed Tomography in a Patient With Acute Gout.