Document Detail

Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.
MedLine Citation:
PMID:  23150439     Owner:  NLM     Status:  Publisher    
Protein-protein interaction studies can provide valuable insight into protein function. One of the most practical and high-yielding approaches is immunoprecipitation of a bait protein followed by mass spectrometry to identify co-precipitating proteins. Here we describe an effective and simplified version of this method that can be performed in most laboratories using standard laboratory equipment (apart from the mass spectrometer). We further demonstrate the utility of this method to identify proteins that specifically interact with mutant forms of the tumor suppressor protein, p53.
Sumitra Deb; Paul R Graves
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Publication Detail:
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  962     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  85-94     Citation Subset:  -    
Department of Biochemistry, Massey Cancer Center, Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA,
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