| Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis. | |
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MedLine Citation:
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PMID: 23150439 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Protein-protein interaction studies can provide valuable insight into protein function. One of the most practical and high-yielding approaches is immunoprecipitation of a bait protein followed by mass spectrometry to identify co-precipitating proteins. Here we describe an effective and simplified version of this method that can be performed in most laboratories using standard laboratory equipment (apart from the mass spectrometer). We further demonstrate the utility of this method to identify proteins that specifically interact with mutant forms of the tumor suppressor protein, p53. |
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Authors:
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Sumitra Deb; Paul R Graves |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 962 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: 85-94 Citation Subset: - |
Affiliation:
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Department of Biochemistry, Massey Cancer Center, Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond, VA, USA, sdeb@vcu.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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