| Identification of novel mouse genes conferring posthypoxic pauses. | |
| | |
MedLine Citation:
|
PMID: 22539170 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Although central to the susceptibility of adult diseases characterized by abnormal rhythmogenesis, characterizing the genes involved is a challenge. We took advantage of the C57BL/6J (B6) trait of hypoxia-induced periodic breathing and its absence in the C57BL/6J-Chr 1(A/J)/NaJ chromosome substitution strain to test the feasibility of gene discovery for this abnormality. Beginning with a genetic and phenotypic analysis of an intercross study between these strains, we discovered three quantitative trait loci (QTLs) on mouse chromosome 1, with phenotypic effects. Fine-mapping reduced the genomic intervals and gene content, and the introgression of one QTL region back onto the C57BL/6J-Chr 1(A/J)/NaJ restored the trait. mRNA expression of non-synonymous genes in the introgressed region in the medulla and pons found evidence for differential expression of three genes, the highest of which was apolipoprotein A2, a lipase regulator; the apo a2 peptide fragment (THEQLTPLVR), highly expressed in the liver, was expressed in low amounts in the medulla but did not correlate with trait expression. This work directly demonstrates the impact of elements on mouse chromosome 1 in respiratory rhythmogenesis. |
| | |
Authors:
|
C Barton Gillombardo; Motoo Yamauchi; Mark D Adams; Jesse Dostal; Sam Chai; Michael W Moore; Lucas M Donovan; Fang Han; Kingman P Strohl |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-04-26 |
Journal Detail:
|
Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: 113 ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2012 Jul |
Date Detail:
|
Created Date: 2012-07-03 Completed Date: 2012-12-07 Revised Date: 2013-04-16 |
Medline Journal Info:
|
Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 167-74 Citation Subset: IM |
Affiliation:
|
Division of Pulmonary, Critical Care, and Sleep Medicine, University Hospital Case Medical Center and the Louis Stokes Cleveland DVA Medical Center, Case Western Reserve University, Cleveland, Ohio, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apolipoprotein A-II / genetics* Chromosome Mapping Female Male Medulla Oblongata / metabolism Mice Mice, Inbred C57BL Periodicity Pons / metabolism Quantitative Trait Loci* Respiration / genetics* |
| Grant Support | |
ID/Acronym/Agency:
|
NS-052452/NS/NINDS NIH HHS; UL1 RR-024989/RR/NCRR NIH HHS; UL1 RR024989/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Apolipoprotein A-II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: CARDIOVASCULAR EFFECTS OF PARTIAL SLEEP DEPRIVATION IN HEALTHY VOLUNTEERS.
Next Document: LEG INTRAMUSCULAR PRESSURES AND IN VIVO KNEE FORCES DURING LOWER BODY POSITIVE AND NEGATIVE PRESSURE...